Biochem-514, Biochemical Techniques, 3(1-2)
(list of practical)

  1. Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; (4.10)
  2. Prove Beer’s law by creating standard graph between absorbance and concentration; (4.13)
  3.  Demonstration about diffusion, dialysis and osmosis; (2.25, 2.26)
  4. Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; (4.4, 4.8)
  5. Carbohydrate analysis and estimation of glucose by spectrophotometer; (3.9)
  6. Total protein estimation by Biuret/Bradford and UV method; (4.10, 4.11, 4.14)
  7. Cholesterol/sterol estimation by LB reagent method; (6.13)
  8. Proximate analysis of foods and feed; (7.1 to 7.11)
  9. Glycogen: Isolation and characterization; (3.16, 3.17)
  10. Electrophoresis of proteins/nucleic acids. (4.16)
Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd  edition, ISBN: 978-969-7502-04-1.

Mid-term syllabus of Biochem-514, 3(1-2):

Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures; Laboratory glassware and basic instrumentation; 

…. PLUS…. First 150 MCQs from chapters 2 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.

 Final syllabus of Biochem-514:

…. PLUS…. 30% of final paper will contain syllabus of mid

…. PLUS…. Standard procedures: Preparation and storage of reagents. Donnan’s equilibrium; Theoretical background of chromatography; Basics of electrophoresis; Importance of biochemical analysis in plant and animal sciences.

…. PLUS…. First 150 MCQs of each of chapter 2,3 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters may also appear in the exam.

 Assignemtns and presentations of Biochem-514: 

  1. The weightage of the assignment and presentation will be decided later. 
  2. Start preparing your presentations and assignments….. Assignment submission is 18-04-2023.
  3. The presentations will also be started right after the mid-term exam.
  4. PPT file should not contain any designing/colours other than figures and diagrams.
  5. Slides should be in 16X9 ratio.
  6. Fonts of all header should be same and of all other text should be same. 
  7. For detailed guidelines about the presentation of research article, visit: https://www.lifesciencesorg.combiochem-720/
  8. Download the template ppt file to make your presentation. The link is here (copy this link and paste in a new tab).
  9. Strictly follow the ppt format and it is better if you write and paste your material in the same ppt file.

NO EXCUSE WILL BE ACCEPTED

 

K. General Question to be asked during presentation

1) Pathway or importance of gene/technology/discovery

2) Rationale

3) Conclusion of the full article

4) Conclusion of every figure

5) New findings presented in the article

6) Applications of the findings

7) Future prospective

8) Applications of the work

9) Principle of every technique used in the research article

Moreover, provide me a printed summary of your article containing the above headings given under ‘K’.

L. During Presentations, for EVERY FIGURE and graph, we may ask:

1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively

__________________________________________________________________________________

Link to download the book ( MCQs in Biochemistry) is given below:

https://b-ok.cc/dl/1055171/2292c5


__________________________________________________________________________________

Microsoft Teams meeting
Join on your computer, mobile app or room device

Click here to join the meeting

Meeting ID: 316 824 288 013
Passcode: WNyjeA

 __________________________________________________________________________________
 

Microsoft teams will be used whenever online classes will be held. You must make your first name as your registration number.

2017-ag-9382 Haidar Iftikhar

2017-ag-10077 Ahmad Faraz 

 

L. During Presentations, for EVERY FIGURE and graph, we may ask:

1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively

  1. BIOCHEM-514        Biochemical Techniques            3(1-2)

 Course Learning Outcomes

By the end of this course students will be able to;

  1. 1.       Demonstrate lab. safety related knowledge and protocols, alongwith general lab tools and instruments
  2. 2.       Relate advance knowledge of chromatography, dialysis, photometry, electrophoresis in the analysis of biomolecules
  3. 3.       Estimate and identify biomolecules via chromatography, colorimeter/spectrophotometer, titrations etc.
  4. 4.       Analyse food and feed samples and learn electrophoresis

Theory

Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures; Laboratory glassware and basic instrumentation; Standard procedures: Preparation and storage of reagents. Donnan’s equilibrium; Theoretical background of chromatography; Basics of electrophoresis; Importance of biochemical analysis in plant and animal sciences.

 

Practical

Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; Prove Beer’s law by creating standard graph between absorbance and concentration; Demonstration about diffusion, dialysis and osmosis; Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; Carbohydrate analysis and estimation of glucose by spectrophotometer; True protein estimation by Biuret/Bradford and UV method; Cholesterol/sterol estimation by LB reagent method; Proximate analysis of foods and feed; Glycogen: Isolation and characterization; Electrophoresis of proteins/nucleic acids.

 

Suggested Readings

  1. 1. Boyer, R.F. 2014. Modern Experimental Biochemistry. 3rd Ed. The Benjamin/Cummings Publishing Co., USA.
  2. 2. Kaushik, G.G., N. Sharma, S. Dabeer and R. Jindal. 2020. Practical Manual of Biochemistry. CBS Publishers & Distributors Pvt Ltd, India.
  3. 3. Sawhney, S.K. and R. Singh. 2014. Introductory Practical Biochemistry. Narosa Publishing House, New Delhi, India.
  4. 4. Shawn, O.F. and L.E. Taylor. 2009. Biochemistry Lab Manual. Cengage Learning, USA.
  5. 5. Varley, H., A.H. Gowenlock, J.R. McMurray and D.M. McLauchlan. 2006. Varley’s Practical Clinical Biochemistry. 6th Ed. CBS Publishers and distributors. New Delhi, India.
  6.  Wilson, K. and J. Walker. 2018. Practical Biochemistry: Principles and Techniques. 8th Ed. Cambridge University Press, UK.
  7. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd  edition, ISBN: 978-969-7502-04-1.
  8. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series II), 2023. 2nd edition, ISBN: 978-969-7502-05-8.
  9. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series III), 2024, 1st edition, ISBN: 978-969-7502-06-5.
Biochem-514, BS Biochem, 6MB-Spring 2023, Dr. Muhammad Ali (30-01-2023 onwards)
Sr.Ag No.Student’s NameGroup #Topic 
12020-ag-8541Amna ZafarG1Integrated Proteogenomic Characterization of Clear Cell Renal Cell Carcinoma1st week after mid
22020-ag-8544Areesha Naeem
32020-ag-8545Arooj Rizwan
42020-ag-8577Mahnoor Rauf
52020-ag-8584Minahil Noor
62020-ag-8587Momina FatimaG2Endocytosis Inhibition in Humans to Improve Responses to ADCC-Mediating Antibodies1st week after mid
72020-ag-8588Muhammad Adnan
82020-ag-8589Muhammad Adnan Zahoor
92020-ag-8590Muhammad Ahmad
102020-ag-8591Muhammad Anwaar Mazhar
112020-ag-8592Muhammad Babar TaimoorG3Lipid-Associated Macrophages Control Metabolic Homeostasis in a Trem2-Dependent Manner1st week after mid
122020-ag-8593Muhammad Hamza Javed
132020-ag-8594Muhammad Mudassar Anwar
142020-ag-8595Muhammad Rizwan Arshad
152020-ag-8596Muhammad Shahzad
162020-ag-8598Muhammad Usman AliG4Coordinate β-adrenergic inhibition of mitochondrial
activity and angiogenesis arrest tumor growth
2nd week after mid
172020-ag-8599Muhammad Zain Sajjad
182020-ag-8600Muizz Shahid
192020-ag-8601Muqadas Fatima
202020-ag-8602Namra Shahzadi
212020-ag-8603Nawal ManzoorG5Protein mimetic amyloid inhibitor potently abrogates cancer-associated mutant p53 aggregation and restores tumor suppressor function2nd week after mid
222020-ag-8604Noor Ul Eman
232020-ag-8606Qurat ul Ain
242020-ag-8608Rimsha Fatima
252020-ag-8609Rimsha Kanwal
262020-ag-8610Sadia HabibG6Rhoifolin from Plumula Nelumbinis exhibits anti cancer efects in pancreatic cancer viaAKT/JNK signaling pathways2nd week after mid
272020-ag-8611Sahr Muneer
282020-ag-8612Saif Ul Rahman
292020-ag-8613Saman Liaqat
302020-ag-8614Saman Saeed
312020-ag-8615Sana SarfrazG7Cell-penetrating Alphabody protein scaffolds for intracellular drug targeting3rd week after mid
322020-ag-8617Sara Nadeem
332020-ag-8618Shumaim Mukhtar
342020-ag-8619Sidra Sagar
352020-ag-8620Soulat Fatima
362020-ag-8621Syeda Sania ZahidG8SREBP-1c impairs ULK1 sulfhydration-mediated autophagic flux to promote hepatic steatosis in high-fat-diet-fed mice3rd week after mid
372020-ag-8622Syeda Sunober Saqlain
382020-ag-8624Tunziha Shabbir
392020-ag-8626Ume Rubab
402020-ag-8627Zainab NoorG9Insulin- and Lipopolysaccharide-Mediated Signaling in Adipose Tissue Macrophages Regulates Postprandial Glycemia through Akt-mTOR Activation3rd week after mid
412020-ag-8628Zeeshan Azam
422020-ag-8629Tuba Majeed
432020-ag-8631Muhammad Usman 
442020-ag-9763Abdullah
Biochem-514, BS Biochem, 6EA-Spring 2023, Dr. Muhammad Ali
Sr.Ag No.Student’s NameGroup #TopicDate
12020-ag-8632Aaima-Tur-RehmanG1Integrated Proteogenomic Characterization of Clear Cell Renal Cell Carcinoma1st week after mid
22020-ag-8633Aleeha Khurram
32020-ag-8634Amna Abbas
42020-ag-8635Amna Batool
52020-ag-8636Aneeq Munsif
62020-ag-8637Aniqa Alwi
72020-ag-8638Areej Fatima KhanG2Endocytosis Inhibition in Humans to Improve Responses to ADCC-Mediating Antibodies1st week after mid
82020-ag-8639Arham Ali
92020-ag-8640Ayesha Rasheed
102020-ag-8641Ayesha Zia
112020-ag-8642Bisma Yousaf
122020-ag-8643Esha Anwar
132020-ag-8644Esha SarwarG3Lipid-Associated Macrophages Control Metabolic Homeostasis in a Trem2-Dependent Manner1st week after mid
142020-ag-8645Faiza Jabeen
152020-ag-8646Hadia Tahir
162020-ag-8647Hamna Chaudhary
172020-ag-8648Hurara Ashraf
182020-ag-8649Ifra Asghar
192020-ag-8650 Ifra AsimG4Coordinate β-adrenergic inhibition of mitochondrial
activity and angiogenesis arrest tumor growth
2nd week after mid
202020-ag-8652Iqra Shahid
212020-ag-8653Khadija Zahra
222020-ag-8654Kinza Sameen
232020-ag-8655Laiba Abrar
242020-ag-8656Laiba Kiran
252020-ag-8657Linta MustafaG5Protein mimetic amyloid inhibitor potently abrogates cancer-associated mutant p53 aggregation and restores tumor suppressor function2nd week after mid
262020-ag-8658Mahnoor Muqadas
272020-ag-8659Maleeha Tariq
282020-ag-8660Moattar Fatima
292020-ag-8661Moazim Rehman
302020-ag-8662Muhammad Awais
312020-ag-8663Muhammad FaizanG6Rhoifolin from Plumula Nelumbinis exhibits anti cancer efects in pancreatic cancer viaAKT/JNK signaling pathways2nd week after mid
322020-ag-8664Muhammad Kashif
332020-ag-8665Muhammad Muneeb Qamar
342020-ag-8666Muhammad Umar
352020-ag-8667Muneeba Naveed
362020-ag-8668Muntaha Fatima
372020-ag-8670Narmeen ZahraG7Cell-penetrating Alphabody protein scaffolds for intracellular drug targeting3rd week after mid
382020-ag-8671Nashra Nadeem
392020-ag-8672Nimra Ahmad
402020-ag-8673Nirma Sarfraz
412020-ag-8674Noor-ul-Huda
422020-ag-8675Noshaba Naz
432020-ag-8676Rida ZainabG8SREBP-1c impairs ULK1 sulfhydration-mediated autophagic flux to promote hepatic steatosis in high-fat-diet-fed mice3rd week after mid
442020-ag-8678Romaan Abeer
452020-ag-8679Sadaf Manzoor
462020-ag-8680Sadia Falak
472020-ag-8681Samra Murtaza
482020-ag-8682Shireen Faisal
492020-ag-8683Sufyan ArifG9Insulin- and Lipopolysaccharide-Mediated Signaling in Adipose Tissue Macrophages Regulates Postprandial Glycemia through Akt-mTOR Activation3rd week after mid
502020-ag-8684Tania Javed
512020-ag-8685Tehreem Ali
522020-ag-8687Uqba Sarfraz
532020-ag-8688Usama Bin Imran
542020-ag-8689Zara Asmat
552020-ag-8690Shaloom Suhail
MMGL
This is template... Try to write, whatever headings are possible for a practical.
Life Sciences by Dr. Muhammad Ali
This is template... Try to write, whatever headings are possible for a practical.

Add your first comment to this post

Comments are closed.