Biochem-514, Biochemical Techniques, 3(1-2)
(list of practical)
- Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; (4.10)
- Prove Beer’s law by creating standard graph between absorbance and concentration; (4.13)
- Demonstration about diffusion, dialysis and osmosis; (2.25, 2.26)
- Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; (4.4, 4.8)
- Carbohydrate analysis and estimation of glucose by spectrophotometer; (3.9)
- Total protein estimation by Biuret/Bradford and UV method; (4.10, 4.11, 4.14)
- Cholesterol/sterol estimation by LB reagent method; (6.13)
- Proximate analysis of foods and feed; (7.1 to 7.11)
- Glycogen: Isolation and characterization; (3.16, 3.17)
- Electrophoresis of proteins/nucleic acids. (4.16)
Mid-term syllabus of Biochem-514, 3(1-2):
Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures; Laboratory glassware and basic instrumentation; …….PLUS….. experiment 2.1 (how will you prepare 50 mL of 0.2 molar solution of glucose?).
…. PLUS…. First 150 MCQs from chapters 2 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.
Final syllabus of Biochem-514:
…. PLUS…. 30% of final paper will contain syllabus of mid
…. PLUS…. Standard procedures: Preparation and storage of reagents. Donnan’s equilibrium; Theoretical background of chromatography; Basics of electrophoresis; Importance of biochemical analysis in plant and animal sciences.
…. PLUS…. First 150 MCQs of each of chapter 2,3 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters may also appear in the exam.
Assignemtns and presentations of Biochem-514:
- The weightage of the assignment and presentation will be decided later.
- Start preparing your presentations and assignments….. Assignment submission is 18-04-2023.
- The presentations will also be started right after the mid-term exam.
- PPT file should not contain any designing/colours other than figures and diagrams.
- Slides should be in 16X9 ratio.
- Fonts of all header should be same and of all other text should be same.
- For detailed guidelines about the presentation of research article, visit: https://www.lifesciencesorg.combiochem-720/
- Download the template ppt file to make your presentation. The link is here (copy this link and paste in a new tab).
- Strictly follow the ppt format and it is better if you write and paste your material in the same ppt file.
NO EXCUSE WILL BE ACCEPTED
K. General Question to be asked during presentation
1) Pathway or importance of gene/technology/discovery
2) Rationale
3) Conclusion of the full article
4) Conclusion of every figure
5) New findings presented in the article
6) Applications of the findings
7) Future prospective
8) Applications of the work
9) Principle of every technique used in the research article
Moreover, provide me a printed summary of your article containing the above headings given under ‘K’.
L. During Presentations, for EVERY FIGURE and graph, we may ask:
1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively
__________________________________________________________________________________
Link to download the book ( MCQs in Biochemistry) is given below:
https://b-ok.cc/dl/1055171/2292c5
__________________________________________________________________________________
Meeting ID: 316 824 288 013
Passcode: WNyjeA
Microsoft teams will be used whenever online classes will be held. You must make your first name as your registration number.
2017-ag-9382 Haidar Iftikhar
2017-ag-10077 Ahmad Faraz
L. During Presentations, for EVERY FIGURE and graph, we may ask:
1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively
- BIOCHEM-514 Biochemical Techniques 3(1-2)
Course Learning Outcomes
By the end of this course students will be able to;
- 1. Demonstrate lab. safety related knowledge and protocols, alongwith general lab tools and instruments
- 2. Relate advance knowledge of chromatography, dialysis, photometry, electrophoresis in the analysis of biomolecules
- 3. Estimate and identify biomolecules via chromatography, colorimeter/spectrophotometer, titrations etc.
- 4. Analyse food and feed samples and learn electrophoresis
Theory
Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures; Laboratory glassware and basic instrumentation; Standard procedures: Preparation and storage of reagents. Donnan’s equilibrium; Theoretical background of chromatography; Basics of electrophoresis; Importance of biochemical analysis in plant and animal sciences.
Practical
Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; Prove Beer’s law by creating standard graph between absorbance and concentration; Demonstration about diffusion, dialysis and osmosis; Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; Carbohydrate analysis and estimation of glucose by spectrophotometer; True protein estimation by Biuret/Bradford and UV method; Cholesterol/sterol estimation by LB reagent method; Proximate analysis of foods and feed; Glycogen: Isolation and characterization; Electrophoresis of proteins/nucleic acids.
Suggested Readings
- 1. Boyer, R.F. 2014. Modern Experimental Biochemistry. 3rd Ed. The Benjamin/Cummings Publishing Co., USA.
- 2. Kaushik, G.G., N. Sharma, S. Dabeer and R. Jindal. 2020. Practical Manual of Biochemistry. CBS Publishers & Distributors Pvt Ltd, India.
- 3. Sawhney, S.K. and R. Singh. 2014. Introductory Practical Biochemistry. Narosa Publishing House, New Delhi, India.
- 4. Shawn, O.F. and L.E. Taylor. 2009. Biochemistry Lab Manual. Cengage Learning, USA.
- 5. Varley, H., A.H. Gowenlock, J.R. McMurray and D.M. McLauchlan. 2006. Varley’s Practical Clinical Biochemistry. 6th Ed. CBS Publishers and distributors. New Delhi, India.
- Wilson, K. and J. Walker. 2018. Practical Biochemistry: Principles and Techniques. 8th Ed. Cambridge University Press, UK.
- Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd edition, ISBN: 978-969-7502-04-1.
- Ali, M. et al., Experimental Biochemistry and Biotechnology (Series II), 2023. 2nd edition, ISBN: 978-969-7502-05-8.
- Ali, M. et al., Experimental Biochemistry and Biotechnology (Series III), 2024, 1st edition, ISBN: 978-969-7502-06-5.
| Biochem-514-6MA, BS Biochem, MA- Spring 2026, Dr. Muhammad Ali; 09-02-2026 (only practical) | |||||
| Sr. | Ag No. | Student’s Name | Assignment for group discussion and for making PPT according to template, one page summary and answers of 9 questions and presentation in the classResearch article for presentation and 1 page summary | ||
| 1 | 2020-ag-9650 | MUHAMMAD ZAEEM KHAN LODHI | G1 | Article 28 | 1. Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; (4.10) |
| 2 | 2023-ag-8497 | AYEZA ABBAS | |||
| 3 | 2023-ag-8498 | MISBAH IMTIAZ | |||
| 4 | 2023-ag-8499 | MAH JABEEN | |||
| 5 | 2023-ag-8500 | LAIBA MOHSIN | 2. Prove Beer’s law by creating standard graph between absorbance and concentration; (4.13) | ||
| 6 | 2023-ag-8501 | LAIBA IKRAM | |||
| 7 | 2023-ag-8502 | SEHRISH AKBAR | |||
| 8 | 2023-ag-8503 | INFAL NASIR | 3. Demonstration about diffusion, dialysis and osmosis; (2.25, 2.26) | ||
| 9 | 2023-ag-8504 | ASMA ALTAF | |||
| 10 | 2023-ag-8505 | IMAN NASIR | |||
| 11 | 2023-ag-8506 | ANEEZA FATIMA | G2 | Article 29 | |
| 12 | 2023-ag-8507 | NOOR FATIMA IDREES | |||
| 13 | 2023-ag-8508 | LAIBA ALI | 4. Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; (4.4, 4.8) | ||
| 14 | 2023-ag-8509 | EMAN KANWAL | |||
| 15 | 2023-ag-8510 | AREEBA MALIK | |||
| 16 | 2023-ag-8511 | TANZEELA AMEEN | |||
| 17 | 2023-ag-8512 | NOOR FATIMA | 5. Carbohydrate analysis and estimation of glucose by spectrophotometer; (3.9) | ||
| 18 | 2023-ag-8513 | HALEEMA AMIR | |||
| 19 | 2023-ag-8514 | ZUHAA IMRAN | |||
| 20 | 2023-ag-8515 | MAIDA MANZOOR | |||
| 21 | 2023-ag-8516 | FARWA KAMRAN | G3 | Article 30 | 6. Total protein estimation by Biuret/Bradford and UV method; (4.10, 4.11, 4.14) |
| 22 | 2023-ag-8517 | TARBEEA KASAM | |||
| 23 | 2023-ag-8518 | VANEEZA SALEEM | |||
| 24 | 2023-ag-8519 | NABIHA MAJEED | |||
| 25 | 2023-ag-8520 | ROBINA SAJJAD | |||
| 26 | 2023-ag-8521 | KIRAN IRSHAD | 7. Cholesterol/sterol estimation by LB reagent method; (6.13) | ||
| 27 | 2023-ag-8522 | AARFA | |||
| 28 | 2023-ag-8523 | HINA FATIMA | |||
| 29 | 2023-ag-8525 | TAHREEM ASGHAR | |||
| 30 | 2023-ag-8526 | AMNA AHMAD | |||
| 31 | 2023-ag-8527 | ARFA RIAZ | G4 | Article 4 | 8. Proximate analysis of foods and feed; (7.1 to 7.11) |
| 32 | 2023-ag-8528 | AENA ABUBAKAR | |||
| 33 | 2023-ag-8529 | AMNA AMEEN | |||
| 34 | 2023-ag-8530 | MOMNA SHAHZAD | |||
| 35 | 2023-ag-8531 | HUMNA EJAZ | |||
| 36 | 2023-ag-8532 | SEHAR ANDLEEB ASLAM | |||
| 37 | 2023-ag-8534 | AQSA ASHIQ | 9. Glycogen: Isolation and characterization; (3.16, 3.17) | ||
| 38 | 2023-ag-8535 | MARIA EMAN ZAHID | |||
| 39 | 2023-ag-8536 | HAMNA IDREES | |||
| 40 | 2023-ag-8537 | MISBAH AZHAR | |||
| 41 | 2023-ag-8538 | AFIA NISAR | G5 | Article 5 | |
| 42 | 2023-ag-8539 | FATIMA IHSAN | |||
| 43 | 2023-ag-8540 | QURAT-UL-AIN | 10. Electrophoresis of proteins/nucleic acids. (4.16) | ||
| 44 | 2023-ag-8541 | SEHRISH BATOOL | |||
| 45 | 2023-ag-8542 | MEMOONA BIBI | |||
| 46 | 2023-ag-8543 | DOSHAL LIAQAT | |||
| 47 | 2023-ag-8544 | TRABI TARA | |||
| 48 | 2023-ag-8545 | TAYYABA SAGHEER | |||
| 49 | 2023-ag-8546 | LAIBA SAEED | 1. Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; (4.10) | ||
| 50 | 2023-ag-8547 | RIMSHA | |||
| 51 | 2023-ag-8548 | PAKIZA ASHFAQ | G6 | Article 6 | |
| 52 | 2023-ag-8549 | FARWA AKRAM | |||
| 53 | 2023-ag-8550 | MALAIKA ALTAF | 2. Prove Beer’s law by creating standard graph between absorbance and concentration; (4.13) | ||
| 54 | 2023-ag-8551 | SADAF NAZ | |||
| 55 | 2023-ag-8552 | ABEEHA ATEEQ | |||
| 56 | 2023-ag-8553 | RAHIMA SAKHAWAT | 3. Demonstration about diffusion, dialysis and osmosis; (2.25, 2.26) | ||
| 57 | 2023-ag-8554 | RABECCA IHSAN | |||
| 58 | 2023-ag-8555 | SADIA MUSKAN | |||
| 59 | 2023-ag-8556 | MAHAM YOUSAF | |||
| 60 | 2023-ag-8557 | NOOR FATIMA | |||
| 61 | 2023-ag-8558 | FAJAR REHMAN | G7 | Article 7 | 4. Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; (4.4, 4.8) |
| 62 | 2023-ag-8559 | MARYAM YOUSAF | |||
| 63 | 2023-ag-8560 | ABEEHA ASGHAR | |||
| 64 | 2023-ag-8561 | ZARIA TAYYAB | |||
| 65 | 2023-ag-8562 | AREEBA NADEEM AKHTAR | 5. Carbohydrate analysis and estimation of glucose by spectrophotometer; (3.9) | ||
| 66 | 2023-ag-8564 | UME HABIBA | |||
| 67 | 2023-ag-8565 | MAHNOOR ZAKIR | |||
| 68 | 2023-ag-8566 | SABA ZAFAR | |||
| 69 | 2023-ag-8567 | FAIQA | 6. Total protein estimation by Biuret/Bradford and UV method; (4.10, 4.11, 4.14) | ||
| 70 | 2023-ag-8568 | HIRA AKBAR | |||
| 71 | 2023-ag-8569 | AMARA | G8 | Article 8 | |
| 72 | 2023-ag-8570 | NOOR FATIMA | |||
| 73 | 2023-ag-8571 | MUHAMMAD HASNAIN | |||
| 74 | 2023-ag-8573 | MUHAMMAD AKASH | 7. Cholesterol/sterol estimation by LB reagent method; (6.13) | ||
| 75 | 2023-ag-8574 | AREEBA | |||
| 76 | 2023-ag-8575 | JAVERIA | |||
| 77 | 2023-ag-8576 | MARYAM YOUSAF | |||
| 78 | 2023-ag-8577 | FIZA | |||
| 79 | 2023-ag-8578 | IQRA FATIMA | 8. Proximate analysis of foods and feed; (7.1 to 7.11) | ||
| 80 | 2023-ag-8579 | TEHREEM | |||
| 81 | 2023-ag-8580 | HIJAB NADEEM | G9 | Article 9 | |
| 82 | 2023-ag-8584 | YASHAL TANVEER | |||
| 83 | 2023-ag-8585 | SYEDA SHEHREEN FATIMA | |||
| 84 | 2023-ag-8586 | WANIA FATIMA | |||
| 85 | 2023-ag-8587 | SADAF BIBI | 9. Glycogen: Isolation and characterization; (3.16, 3.17) | ||
| 86 | 2023-ag-8588 | RABIA JAMAL | |||
| 87 | 2023-ag-8589 | HAMMAD HUSSAIN | |||
| 88 | 2023-ag-8590 | EMAN FATIMA | |||
| 89 | 2023-ag-8591 | NIMRA AHMAD | |||
| 90 | 2023-ag-8592 | AYESHA BIBI | |||
| 91 | 2023-ag-8593 | FASIH UR REHMAN | G10 | Article 10 | 10. Electrophoresis of proteins/nucleic acids. (4.16) |
| 92 | 2023-ag-8594 | MUNTAHA SAJJAD | |||
| 93 | 2023-ag-8595 | SADIA KANWAL | |||
| 94 | 2023-ag-8596 | MUQADAS FATIMA | |||
| 95 | 2023-ag-8597 | AMINA IQBAL | |||
| 96 | 2023-ag-8598 | MINAHIL | |||
| 97 | 2023-ag-8600 | SHAHZAIB NASIR | |||
