Biochem-514, Biochemical Techniques, 3(1-2)
(list of practical)

  1. Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; (4.10)
  2. Prove Beer’s law by creating standard graph between absorbance and concentration; (4.13)
  3.  Demonstration about diffusion, dialysis and osmosis; (2.25, 2.26)
  4. Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; (4.4, 4.8)
  5. Carbohydrate analysis and estimation of glucose by spectrophotometer; (3.9)
  6. Total protein estimation by Biuret/Bradford and UV method; (4.10, 4.11, 4.14)
  7. Cholesterol/sterol estimation by LB reagent method; (6.13)
  8. Proximate analysis of foods and feed; (7.1 to 7.11)
  9. Glycogen: Isolation and characterization; (3.16, 3.17)
  10. Electrophoresis of proteins/nucleic acids. (4.16)
Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd  edition, ISBN: 978-969-7502-04-1.

Mid-term syllabus of Biochem-514, 3(1-2):

Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures; Laboratory glassware and basic instrumentation; …….PLUS….. experiment 2.1 (how will you prepare 50 mL of 0.2 molar solution of glucose?).

…. PLUS…. First 150 MCQs from chapters 2 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.

 Final syllabus of Biochem-514:

…. PLUS…. 30% of final paper will contain syllabus of mid

…. PLUS…. Standard procedures: Preparation and storage of reagents. Donnan’s equilibrium; Theoretical background of chromatography; Basics of electrophoresis; Importance of biochemical analysis in plant and animal sciences.

…. PLUS…. First 150 MCQs of each of chapter 2,3 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters may also appear in the exam.

 Assignemtns and presentations of Biochem-514: 

  1. The weightage of the assignment and presentation will be decided later. 
  2. Start preparing your presentations and assignments….. Assignment submission is 18-04-2023.
  3. The presentations will also be started right after the mid-term exam.
  4. PPT file should not contain any designing/colours other than figures and diagrams.
  5. Slides should be in 16X9 ratio.
  6. Fonts of all header should be same and of all other text should be same. 
  7. For detailed guidelines about the presentation of research article, visit: https://www.lifesciencesorg.combiochem-720/
  8. Download the template ppt file to make your presentation. The link is here (copy this link and paste in a new tab).
  9. Strictly follow the ppt format and it is better if you write and paste your material in the same ppt file.

NO EXCUSE WILL BE ACCEPTED

 

K. General Question to be asked during presentation

1) Pathway or importance of gene/technology/discovery

2) Rationale

3) Conclusion of the full article

4) Conclusion of every figure

5) New findings presented in the article

6) Applications of the findings

7) Future prospective

8) Applications of the work

9) Principle of every technique used in the research article

Moreover, provide me a printed summary of your article containing the above headings given under ‘K’.

L. During Presentations, for EVERY FIGURE and graph, we may ask:

1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively

__________________________________________________________________________________

Link to download the book ( MCQs in Biochemistry) is given below:

https://b-ok.cc/dl/1055171/2292c5


__________________________________________________________________________________

Microsoft Teams meeting
Join on your computer, mobile app or room device

Click here to join the meeting

Meeting ID: 316 824 288 013
Passcode: WNyjeA

 __________________________________________________________________________________
 

Microsoft teams will be used whenever online classes will be held. You must make your first name as your registration number.

2017-ag-9382 Haidar Iftikhar

2017-ag-10077 Ahmad Faraz 

 

L. During Presentations, for EVERY FIGURE and graph, we may ask:

1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively

  1. BIOCHEM-514        Biochemical Techniques            3(1-2)

 Course Learning Outcomes

By the end of this course students will be able to;

  1. 1.       Demonstrate lab. safety related knowledge and protocols, alongwith general lab tools and instruments
  2. 2.       Relate advance knowledge of chromatography, dialysis, photometry, electrophoresis in the analysis of biomolecules
  3. 3.       Estimate and identify biomolecules via chromatography, colorimeter/spectrophotometer, titrations etc.
  4. 4.       Analyse food and feed samples and learn electrophoresis

Theory

Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures; Laboratory glassware and basic instrumentation; Standard procedures: Preparation and storage of reagents. Donnan’s equilibrium; Theoretical background of chromatography; Basics of electrophoresis; Importance of biochemical analysis in plant and animal sciences.

 

Practical

Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; Prove Beer’s law by creating standard graph between absorbance and concentration; Demonstration about diffusion, dialysis and osmosis; Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; Carbohydrate analysis and estimation of glucose by spectrophotometer; True protein estimation by Biuret/Bradford and UV method; Cholesterol/sterol estimation by LB reagent method; Proximate analysis of foods and feed; Glycogen: Isolation and characterization; Electrophoresis of proteins/nucleic acids.

 

Suggested Readings

  1. 1. Boyer, R.F. 2014. Modern Experimental Biochemistry. 3rd Ed. The Benjamin/Cummings Publishing Co., USA.
  2. 2. Kaushik, G.G., N. Sharma, S. Dabeer and R. Jindal. 2020. Practical Manual of Biochemistry. CBS Publishers & Distributors Pvt Ltd, India.
  3. 3. Sawhney, S.K. and R. Singh. 2014. Introductory Practical Biochemistry. Narosa Publishing House, New Delhi, India.
  4. 4. Shawn, O.F. and L.E. Taylor. 2009. Biochemistry Lab Manual. Cengage Learning, USA.
  5. 5. Varley, H., A.H. Gowenlock, J.R. McMurray and D.M. McLauchlan. 2006. Varley’s Practical Clinical Biochemistry. 6th Ed. CBS Publishers and distributors. New Delhi, India.
  6.  Wilson, K. and J. Walker. 2018. Practical Biochemistry: Principles and Techniques. 8th Ed. Cambridge University Press, UK.
  7. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd  edition, ISBN: 978-969-7502-04-1.
  8. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series II), 2023. 2nd edition, ISBN: 978-969-7502-05-8.
  9. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series III), 2024, 1st edition, ISBN: 978-969-7502-06-5.
Biochem-514-6MA, BS Biochem, MA- Spring 2026, Dr. Muhammad Ali; 09-02-2026
(only practical)
      
Sr.Ag No.Student’s Name Assignment
for group discussion and for making PPT according to template, one page
summary and answers of 9 questions and presentation in the classResearch
article for presentation and 1 page summary
 
12020-ag-9650MUHAMMAD
ZAEEM KHAN LODHI
G1Article
28
1.
Study principles of spectrophotometry/colorimetry by preparing absorption
spectra of any given sample; (4.10)
22023-ag-8497AYEZA
ABBAS
32023-ag-8498MISBAH
IMTIAZ
42023-ag-8499MAH
JABEEN
52023-ag-8500LAIBA
MOHSIN
2.
Prove Beer’s law by creating standard graph between absorbance and
concentration; (4.13)
62023-ag-8501LAIBA
IKRAM
72023-ag-8502SEHRISH
AKBAR
82023-ag-8503INFAL
NASIR
3.
 Demonstration about diffusion, dialysis and osmosis; (2.25, 2.26)
92023-ag-8504ASMA
ALTAF
102023-ag-8505IMAN
NASIR
112023-ag-8506ANEEZA
FATIMA
G2Article
29
122023-ag-8507NOOR
FATIMA IDREES
132023-ag-8508LAIBA
ALI
4.
Chromatography: Paper chromatography, one dimensional two dimensional,
circular and column chromatography; (4.4, 4.8)
142023-ag-8509EMAN
KANWAL
152023-ag-8510AREEBA
MALIK
162023-ag-8511TANZEELA
AMEEN
172023-ag-8512NOOR
FATIMA
5.
Carbohydrate analysis and estimation of glucose by spectrophotometer; (3.9)
182023-ag-8513HALEEMA
AMIR
192023-ag-8514ZUHAA
IMRAN
202023-ag-8515MAIDA
MANZOOR
212023-ag-8516FARWA
KAMRAN
G3Article
30
6.
Total protein estimation by Biuret/Bradford and UV method; (4.10, 4.11, 4.14)
222023-ag-8517TARBEEA
KASAM
232023-ag-8518VANEEZA
SALEEM
242023-ag-8519NABIHA
MAJEED
252023-ag-8520ROBINA
SAJJAD
262023-ag-8521KIRAN
IRSHAD
7.
Cholesterol/sterol estimation by LB reagent method; (6.13)
272023-ag-8522AARFA
282023-ag-8523HINA
FATIMA
292023-ag-8525TAHREEM
ASGHAR
302023-ag-8526AMNA
AHMAD
312023-ag-8527ARFA
RIAZ
G4Article
4
8.
Proximate analysis of foods and feed; (7.1 to 7.11)
322023-ag-8528AENA
ABUBAKAR
332023-ag-8529AMNA
AMEEN
342023-ag-8530MOMNA
SHAHZAD
352023-ag-8531HUMNA
EJAZ
362023-ag-8532SEHAR
ANDLEEB ASLAM
372023-ag-8534AQSA
ASHIQ
9.
Glycogen: Isolation and characterization; (3.16, 3.17)
382023-ag-8535MARIA
EMAN ZAHID
392023-ag-8536HAMNA
IDREES
402023-ag-8537MISBAH
AZHAR
412023-ag-8538AFIA
NISAR
G5Article
5
422023-ag-8539FATIMA
IHSAN
432023-ag-8540QURAT-UL-AIN10.
Electrophoresis of proteins/nucleic acids. (4.16)
442023-ag-8541SEHRISH
BATOOL
452023-ag-8542MEMOONA
BIBI
462023-ag-8543DOSHAL
LIAQAT
472023-ag-8544TRABI
TARA
482023-ag-8545TAYYABA
SAGHEER
492023-ag-8546LAIBA
SAEED
1.
Study principles of spectrophotometry/colorimetry by preparing absorption
spectra of any given sample; (4.10)
502023-ag-8547RIMSHA 
512023-ag-8548PAKIZA
ASHFAQ
G6Article
6
522023-ag-8549FARWA
AKRAM
532023-ag-8550MALAIKA
ALTAF
2.
Prove Beer’s law by creating standard graph between absorbance and
concentration; (4.13)
542023-ag-8551SADAF
NAZ
552023-ag-8552ABEEHA
ATEEQ
562023-ag-8553RAHIMA
SAKHAWAT
3.
 Demonstration about diffusion, dialysis and osmosis; (2.25, 2.26)
572023-ag-8554RABECCA
IHSAN
582023-ag-8555SADIA
MUSKAN
592023-ag-8556MAHAM
YOUSAF
602023-ag-8557NOOR
FATIMA
612023-ag-8558FAJAR
REHMAN
G7Article
7
4.
Chromatography: Paper chromatography, one dimensional two dimensional,
circular and column chromatography; (4.4, 4.8)
622023-ag-8559MARYAM
YOUSAF
632023-ag-8560ABEEHA
ASGHAR
642023-ag-8561ZARIA
TAYYAB
652023-ag-8562AREEBA
NADEEM AKHTAR
5.
Carbohydrate analysis and estimation of glucose by spectrophotometer; (3.9)
662023-ag-8564UME
HABIBA
672023-ag-8565MAHNOOR
ZAKIR
682023-ag-8566SABA
ZAFAR
692023-ag-8567FAIQA6.
Total protein estimation by Biuret/Bradford and UV method; (4.10, 4.11, 4.14)
702023-ag-8568HIRA
AKBAR
712023-ag-8569AMARA G8Article
8
722023-ag-8570NOOR
FATIMA
732023-ag-8571MUHAMMAD
HASNAIN
742023-ag-8573MUHAMMAD
AKASH
7.
Cholesterol/sterol estimation by LB reagent method; (6.13)
752023-ag-8574AREEBA
762023-ag-8575JAVERIA
772023-ag-8576MARYAM
YOUSAF
782023-ag-8577FIZA 
792023-ag-8578IQRA
FATIMA
8.
Proximate analysis of foods and feed; (7.1 to 7.11)
802023-ag-8579TEHREEM 
812023-ag-8580HIJAB
NADEEM
G9Article
9
822023-ag-8584YASHAL
TANVEER
832023-ag-8585SYEDA
SHEHREEN FATIMA
842023-ag-8586WANIA
FATIMA
852023-ag-8587SADAF
BIBI
9.
Glycogen: Isolation and characterization; (3.16, 3.17)
862023-ag-8588RABIA
JAMAL
872023-ag-8589HAMMAD
HUSSAIN
882023-ag-8590EMAN
FATIMA
892023-ag-8591NIMRA
AHMAD
902023-ag-8592AYESHA
BIBI
912023-ag-8593FASIH
UR REHMAN
G10Article
10
10.
Electrophoresis of proteins/nucleic acids. (4.16)
922023-ag-8594MUNTAHA
SAJJAD
932023-ag-8595SADIA
KANWAL
942023-ag-8596MUQADAS
FATIMA 
952023-ag-8597AMINA
IQBAL
962023-ag-8598MINAHIL
972023-ag-8600SHAHZAIB
NASIR
    
    
    
MMGL
This is template... Try to write, whatever headings are possible for a practical.
Life Sciences by Dr. Muhammad Ali
This is template... Try to write, whatever headings are possible for a practical.