Biochem-514, Biochemical Techniques, 3(1-2)
(list of practical)
- Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; (4.10)
- Prove Beer’s law by creating standard graph between absorbance and concentration; (4.13)
- Demonstration about diffusion, dialysis and osmosis; (2.25, 2.26)
- Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; (4.4, 4.8)
- Carbohydrate analysis and estimation of glucose by spectrophotometer; (3.9)
- Total protein estimation by Biuret/Bradford and UV method; (4.10, 4.11, 4.14)
- Cholesterol/sterol estimation by LB reagent method; (6.13)
- Proximate analysis of foods and feed; (7.1 to 7.11)
- Glycogen: Isolation and characterization; (3.16, 3.17)
- Electrophoresis of proteins/nucleic acids. (4.16)
Mid-term syllabus of Biochem-514, 3(1-2):
Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures; Laboratory glassware and basic instrumentation; …….PLUS….. experiment 2.1 (how will you prepare 50 mL of 0.2 molar solution of glucose?).
…. PLUS…. First 150 MCQs from chapters 2 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.
Final syllabus of Biochem-514:
…. PLUS…. 30% of final paper will contain syllabus of mid
…. PLUS…. Standard procedures: Preparation and storage of reagents. Donnan’s equilibrium; Theoretical background of chromatography; Basics of electrophoresis; Importance of biochemical analysis in plant and animal sciences.
…. PLUS…. First 150 MCQs of each of chapter 2,3 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters may also appear in the exam.
Assignemtns and presentations of Biochem-514:
- The weightage of the assignment and presentation will be decided later.
- Start preparing your presentations and assignments….. Assignment submission is 18-04-2023.
- The presentations will also be started right after the mid-term exam.
- PPT file should not contain any designing/colours other than figures and diagrams.
- Slides should be in 16X9 ratio.
- Fonts of all header should be same and of all other text should be same.
- For detailed guidelines about the presentation of research article, visit: https://www.lifesciencesorg.combiochem-720/
- Download the template ppt file to make your presentation. The link is here (copy this link and paste in a new tab).
- Strictly follow the ppt format and it is better if you write and paste your material in the same ppt file.
NO EXCUSE WILL BE ACCEPTED
K. General Question to be asked during presentation
1) Pathway or importance of gene/technology/discovery
2) Rationale
3) Conclusion of the full article
4) Conclusion of every figure
5) New findings presented in the article
6) Applications of the findings
7) Future prospective
8) Applications of the work
9) Principle of every technique used in the research article
Moreover, provide me a printed summary of your article containing the above headings given under ‘K’.
L. During Presentations, for EVERY FIGURE and graph, we may ask:
1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively
__________________________________________________________________________________
Link to download the book ( MCQs in Biochemistry) is given below:
https://b-ok.cc/dl/1055171/2292c5
__________________________________________________________________________________
Meeting ID: 316 824 288 013
Passcode: WNyjeA
Microsoft teams will be used whenever online classes will be held. You must make your first name as your registration number.
2017-ag-9382 Haidar Iftikhar
2017-ag-10077 Ahmad Faraz
L. During Presentations, for EVERY FIGURE and graph, we may ask:
1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively
- BIOCHEM-514 Biochemical Techniques 3(1-2)
Course Learning Outcomes
By the end of this course students will be able to;
- 1. Demonstrate lab. safety related knowledge and protocols, alongwith general lab tools and instruments
- 2. Relate advance knowledge of chromatography, dialysis, photometry, electrophoresis in the analysis of biomolecules
- 3. Estimate and identify biomolecules via chromatography, colorimeter/spectrophotometer, titrations etc.
- 4. Analyse food and feed samples and learn electrophoresis
Theory
Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures; Laboratory glassware and basic instrumentation; Standard procedures: Preparation and storage of reagents. Donnan’s equilibrium; Theoretical background of chromatography; Basics of electrophoresis; Importance of biochemical analysis in plant and animal sciences.
Practical
Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; Prove Beer’s law by creating standard graph between absorbance and concentration; Demonstration about diffusion, dialysis and osmosis; Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; Carbohydrate analysis and estimation of glucose by spectrophotometer; True protein estimation by Biuret/Bradford and UV method; Cholesterol/sterol estimation by LB reagent method; Proximate analysis of foods and feed; Glycogen: Isolation and characterization; Electrophoresis of proteins/nucleic acids.
Suggested Readings
- 1. Boyer, R.F. 2014. Modern Experimental Biochemistry. 3rd Ed. The Benjamin/Cummings Publishing Co., USA.
- 2. Kaushik, G.G., N. Sharma, S. Dabeer and R. Jindal. 2020. Practical Manual of Biochemistry. CBS Publishers & Distributors Pvt Ltd, India.
- 3. Sawhney, S.K. and R. Singh. 2014. Introductory Practical Biochemistry. Narosa Publishing House, New Delhi, India.
- 4. Shawn, O.F. and L.E. Taylor. 2009. Biochemistry Lab Manual. Cengage Learning, USA.
- 5. Varley, H., A.H. Gowenlock, J.R. McMurray and D.M. McLauchlan. 2006. Varley’s Practical Clinical Biochemistry. 6th Ed. CBS Publishers and distributors. New Delhi, India.
- Wilson, K. and J. Walker. 2018. Practical Biochemistry: Principles and Techniques. 8th Ed. Cambridge University Press, UK.
- Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd edition, ISBN: 978-969-7502-04-1.
- Ali, M. et al., Experimental Biochemistry and Biotechnology (Series II), 2023. 2nd edition, ISBN: 978-969-7502-05-8.
- Ali, M. et al., Experimental Biochemistry and Biotechnology (Series III), 2024, 1st edition, ISBN: 978-969-7502-06-5.
- BIOCHEM-514 Biochemical Techniques 3(1-2)
Course Learning Outcomes
By the end of this course students will be able to;
- 1. Demonstrate lab. safety related knowledge and protocols, alongwith general lab tools and instruments
- 2. Relate advance knowledge of chromatography, dialysis, photometry, electrophoresis in the analysis of biomolecules
- 3. Estimate and identify biomolecules via chromatography, colorimeter/spectrophotometer, titrations etc.
- 4. Analyse food and feed samples and learn electrophoresis
Theory
Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures; Laboratory glassware and basic instrumentation; Standard procedures: Preparation and storage of reagents. Donnan’s equilibrium; Theoretical background of chromatography; Basics of electrophoresis; Importance of biochemical analysis in plant and animal sciences.
Practical
Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; Prove Beer’s law by creating standard graph between absorbance and concentration; Demonstration about diffusion, dialysis and osmosis; Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; Carbohydrate analysis and estimation of glucose by spectrophotometer; True protein estimation by Biuret/Bradford and UV method; Cholesterol/sterol estimation by LB reagent method; Proximate analysis of foods and feed; Glycogen: Isolation and characterization; Electrophoresis of proteins/nucleic acids.
Suggested Readings
- 1. Boyer, R.F. 2014. Modern Experimental Biochemistry. 3rd Ed. The Benjamin/Cummings Publishing Co., USA.
- 2. Kaushik, G.G., N. Sharma, S. Dabeer and R. Jindal. 2020. Practical Manual of Biochemistry. CBS Publishers & Distributors Pvt Ltd, India.
- 3. Sawhney, S.K. and R. Singh. 2014. Introductory Practical Biochemistry. Narosa Publishing House, New Delhi, India.
- 4. Shawn, O.F. and L.E. Taylor. 2009. Biochemistry Lab Manual. Cengage Learning, USA.
- 5. Varley, H., A.H. Gowenlock, J.R. McMurray and D.M. McLauchlan. 2006. Varley’s Practical Clinical Biochemistry. 6th Ed. CBS Publishers and distributors. New Delhi, India.
- Wilson, K. and J. Walker. 2018. Practical Biochemistry: Principles and Techniques. 8th Ed. Cambridge University Press, UK.
- Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd edition, ISBN: 978-969-7502-04-1.
- Ali, M. et al., Experimental Biochemistry and Biotechnology (Series II), 2023. 2nd edition, ISBN: 978-969-7502-05-8.
- Ali, M. et al., Experimental Biochemistry and Biotechnology (Series III), 2024, 1st edition, ISBN: 978-969-7502-06-5.
Biochem-514 (2022 scheme), BS Biochemistry M-Spring 2025, Dr. Muhammad Ali | ||||
Sr. | Ag No. | Student’s Name | Group | Topic of practical and number of practical (ppt and presentation) |
1 | 2022-ag-5240 | Tafseela Amiad | G1 | 1-Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; (4.10) |
2 | 2022-ag-5241 | Alisha | ||
3 | 2022-ag-5242 | Ansa bibi | ||
4 | 2022-ag-5243 | Hadia Umar | ||
5 | 2022-ag-5245 | Ahmad Hassan | ||
6 | 2022-ag-5246 | Esha Arshad | ||
7 | 2022-ag-5247 | Asma Nasir | ||
8 | 2022-ag-5248 | Fariha Nawaz | ||
9 | 2022-ag-5249 | Esha Tahir | G2 | 2-Prove Beer’s law by creating standard graph between absorbance and concentration; (4.13) |
10 | 2022-ag-5251 | Sahfa Sajjad | ||
11 | 2022-ag-5252 | Qirat Razzaq | ||
12 | 2022-ag-5253 | Kashaf Alam | ||
13 | 2022-ag-5254 | Aisha Ali | ||
14 | 2022-ag-5255 | Zainab | ||
15 | 2022-ag-5256 | Bushra Noor | ||
16 | 2022-ag-5257 | Mariyam | ||
17 | 2022-ag-5258 | Iqra batool | G3 | 3-Demonstration about diffusion, dialysis and osmosis; (2.25, 2.26) |
18 | 2022-ag-5259 | Manahil Younis | ||
19 | 2022-ag-5260 | Khuzaima Nadeem | ||
20 | 2022-ag-5261 | Ayesha Naveed | ||
21 | 2022-ag-5262 | Fiza Fatima | ||
22 | 2022-ag-5263 | Vaneeza Ahmad | ||
23 | 2022-ag-5264 | Hafsa Tahira | ||
24 | 2022-ag-5265 | M Muzazamal | ||
25 | 2022-ag-5266 | M Bilal | G4 | 4-Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; (4.4, 4.8) |
26 | 2022-ag-5267 | Abdul Hanan | ||
27 | 2022-ag-5268 | Faiza Laraib | ||
28 | 2022-ag-5269 | Saher Fraz | ||
29 | 2022-ag-5270 | M Usman | ||
30 | 2022-ag-5271 | Manahil Malik | ||
31 | 2022-ag-5272 | Maheen Asif | ||
32 | 2022-ag-5274 | Munim Ali | ||
33 | 2022-ag-5276 | Mubashar Ali | G5 | 5-Carbohydrate analysis and estimation of glucose by spectrophotometer; (3.9) |
34 | 2022-ag-5277 | M Shehbaz Jameel | ||
35 | 2022-ag-5278 | Kisa Fatima | ||
36 | 2022-ag-5279 | M Mohsin Raza | ||
37 | 2022-ag-5280 | Kashaf Fatima | ||
38 | 2022-ag-5281 | Laina Azhar | ||
39 | 2022-ag-5282 | Aneela Shazain | ||
40 | 2022-ag-5283 | Rabia Ramazan | ||
41 | 2022-ag-5284 | Eman Fatima | G6 | 6-Total protein estimation by Biuret/Bradford and UV method; (4.10, 4.11, 4.14) |
42 | 2022-ag-5285 | Marium Saddiqua | ||
43 | 2022-ag-5286 | Ariba | ||
44 | 2022-ag-5287 | Marwa Nadeem | ||
45 | 2022-ag-5288 | Ghulam Hussain | ||
46 | 2022-ag-5289 | Samara Khalid | ||
47 | 2022-ag-5292 | Lubaba Saeed | ||
48 | 2022-ag-5293 | Nimra Eman | G7 | 7-Cholesterol/sterol estimation by LB reagent method; (6.13) |
49 | 2022-ag-5294 | Riza Sikandar | ||
50 | 2022-ag-5295 | Hamna Irshad | ||
51 | 2022-ag-5296 | Maryam Hamda | ||
52 | 2022-ag-5297 | Maryam Rahi | ||
53 | 2022-ag-5298 | Ali | ||
54 | 2022-ag-5299 | Haiqa Akhtar | ||
55 | 2022-ag-5300 | Saha Abbas | ||
56 | 2022-ag-5301 | Abdul Rehman | G8 | 8-Proximate analysis of foods and feed; (7.1 to 7.11) |
57 | 2022-ag-5302 | Aiman Hamid | ||
58 | 2022-ag-5303 | Marium Fatima | ||
59 | 2022-ag-5304 | Malaika Nadeem | ||
60 | 2022-ag-5306 | Saba Javed | ||
61 | 2022-ag-5307 | Marriam Saleem | ||
62 | 2022-ag-5308 | Afsheen Qadri | ||
63 | 2022-ag-5309 | Itrat Fatima | G9 | 9-Glycogen: Isolation and characterization; (3.16, 3.17) |
64 | 2022-ag-5311 | Maha | ||
65 | 2022-ag-5312 | Zeeshan | ||
66 | 2022-ag-5313 | Farwa Asghar | ||
67 | 2022-ag-5314 | Umer Akmal | ||
68 | 2022-ag-5315 | Tayyaba Eman | ||
69 | 2022-ag-5316 | Daniya Pakeeza | ||
70 | 2022-ag-5317 | Tayyaba Ali | ||
71 | 2022-ag-5318 | Aiman Shabir | G10 | 10-Electrophoresis of proteins/nucleic acids. (4.16) |
72 | 2022-ag-5319 | Tayyaba Naveed | ||
73 | 2022-ag-7031 | Ayesha Aslam | ||
74 | 2022-ag-5365 | Sabhat | ||
75 | 2022-ag-5355 | Ifra | ||
76 | 2022-ag-5320 | Fatima Idrees | ||
77 | 2022-ag-5338 | Aleeza | ||
78 | 2022-ag-5364 | Fatima Meraj |


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