Biochem-301 (list of practical)

  1. pH metry and determination of pH value of biological fluids. (Will make observations)
  2. Preparation of buffers of definite pH. (2.1, 2.3, 2.7, 2.8, 2.9, 2.19, 2.22)
  3. Estimation of optical activity by polarimetry. (3.10-, 3.11)
  4. Qualitative analysis of carbohydrates. (3.1- 3.8)
  5. Qualitative analysis of urine for normal and abnormal constituents like albumins, acetone bodies and sugar. (I will provide)
  6. Estimation of glucose in biological fluids. (3.9, 3.10)
  7. Determination of acid value, (6.3)
  8. Saponification value (6.5)
  9. and iodine values of fats/oils. (6.6)
  10. Estimation of lactose and casein in milk. (7.11)

Mid-term syllabus of Biochem-301: A general introduction to the science of biochemistry and its importance. Ionization of water, weak acid and weak bases, Define and describe pH, buffers, diffusion, osmosis and osmotic pressure. Enzymes: Classification, nomenclature, characteristics, coenzymes, cofactors and prosthetic groups. Mechanism of enzyme action. Enzyme inhibition. Carbohydrates: Classification, characteristics, aerobic and anaerobic oxidation of glucose; glycolysis, citric acid cycle, Electron transport chain (ETC) and fermentations, biological functions of carbohydrates.

…. PLUS…. 150 MCQs from each of chapters 2 (150) and 6 (150) of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.

Final exam Biochem-301: Lipids: Composition and classification, structure of saturated and unsaturated fatty acids and their properties, characteristics of fats and oils, general metabolism of fats and oils. Proteins: Composition and classification, characteristics and classification of amino acids, peptides and level of structural organization of proteins, physiological function and general metabolism of proteins. Nucleic acids: Chemical composition, structure of DNA and RNA and its types. A general overview of central dogma of molecular biology.

 …. PLUS…. 150 MCQs from chapters 3 (150), 4 (150), 9 (150), 2 (150), 6 (150) of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.

Start preparing your presentations and assignments….. Assignment submission is the second week of next month, October 2023. The presentations will also be started after mid-term exam.

 

K. General Question to be asked during presentation

  1. Pathway or importance of gene/technology/discovery
  2. Rationale
  3. Conclusion of the full article
  4. Conclusion of every figure
  5. New findings presented in the article
  6. Applications of the findings
  7. Future prospective
  8. Applications of the work
  9. Principle of every technique used in the research article

Moreover, provide me a printed summary of your article containing the above headings given under ‘K’.

L. During Presentations, for EVERY FIGURE and graph, we may ask:

1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively

NO EXCUSE WILL BE ACCEPTED

__________________________________________________________________________________

Presentations of Biochem-301: 

  1. The weightage of the assignment and presentation will be decided later. 
  2. Start preparing your presentations……
  3. The presentations will also be started right after the mid-term exam.
  4. PPT file should not contain any designing/colours other than figures and diagrams.
  5. Slides should be in 16X9 ratio.
  6. Fonts of all header should be same and of all other text should be same. 
  7. For detailed guidelines about the presentation of research article, visit: https://www.lifesciencesorg.combiochem-720/
  8. Download the template ppt file to make your presentation. The link is here (copy this link and paste in a new tab).

Some important questions to be asked from students during presentation

  1. 1)       Pathway or importance of gene/technology/drug
  2. 2)       Rationale (justification of the project in this paper)
  3. 3)       Conclusion of every figure
  4. 4)       Conclusion of full article
  5. 5)       New findings present in the article
  6. 6)       Applications of the finding/s.
  7. 7)       Future prospective
  8. 8)       Principle of every technique used in the research article

NO EXCUSE WILL BE ACCEPTED

 _______________________________________________________________

Link to download the book ( MCQs in Biochemistry) is here.


_______________________________________________________________
 
Microsoft Teams meeting
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Meeting ID: 316 824 288 013
Passcode: WNyjeA

 __________________________________________________________________________________
 

Microsoft teams will be used whenever online classes will be held. You must make your first name as your registration number.

2017-ag-9382 Haidar Iftikhar

2017-ag-10077 Ahmad Faraz 

Biochem-301, BS HND, E-Winter 2023-24, Dr. Muhammad Ali

Sr. Ag No. Student’s Name Group Research article for presentation and 1 page summary
1 AYSHA MUSHTAQ AHMED G1 Emodin attenuates high lipid-induced liver metastasis through the AKT and ERK pathways in vitro in breast cancer cells and in a mouse xenograft model
2 SADIA TANVEER MUHAMMAD TANVEER ANWAR SIDHU
3 KHADIJA FAEOOQ FAROOQ AHMAD
4 MARIA SALEEM MUHAMMAD SALEEM
5 TAIBA GHAFFAR ABDUL GHAFFAR
6 NATASHA IRFAN MUHAMMAD IRFAN
7 HAFIZA MARYAM MUHAMMAD RIZWAN
8 AMNA TARIQ TARIQ MEHMOOD G2 PRMT6 Regulates RAS/RAF Binding and MEK/ERKMediated Cancer Stemness Activities in Hepatocellular Carcinoma through CRAF Methylation
9 M HANZALA SHEHZAD ATIF SHEHZAD
10 RIMSHAH QAMAR HABIB QAMAR
11 ROHMA SHARAZ SHARAZ AKHTER SINDHU
12 EMAN FATIMA ASGHAR ALI
13 EMAN FATIMA SHAHID MEHMOOD
14 HASSAN RAZA IRFAN UL HAQ
15 SAYEEDA HAFSA SYED MAQSOOD HUSSAIN SHAH G3 Impaired LXRa Phosphorylation Attenuates Progression of Fatty Liver Disease
16 ASHMAL MAHEEN SHABBIR AHMAD
17 HAFIZA MUNTAHA KHALID MUHAMMAD KHALID
18 MEMOONA MEHMOOD KHALID MEHMOOD
19 MALAIKA ARFAQ ARFAQ AHMAD
20 MISBAH NOREEN MUHAMMAD SHAHID IQBAL
21 EMAN FATIMA MUHAMMAD AFZAL
22 AISHA FATIMA UMER FAROOQ G4 Aldolase B-Mediated Fructose Metabolism Drives Metabolic Reprogramming of Colon Cancer Liver Metastasis
23 AYESHA MUHAMMAD HAFEEZ
24 EMAN AFZAL MUHAMMAD AFZAL ZAHID
25 WISHAH ZAINAB QAMAR ABBAS
26 LAIBA NOOR MUHAMMAD AFZAL
27 JAVERIA BIBI FAIZ ULLAH
28 SARA IJAZ IJAZ AHMAD CHATTHA
29 HAMNA AZEEM MUHAMMAD AKRAM G5 Treatment of acromegaly by rosiglitazone via upregulating 15-PGDH in both pituitary adenoma and liver
30 EESHA HUSSAIN NASIR HUSSAIN
31 MAHEEN MAJID MAJID MUNIR
32 SEERAT FATIMA JAVEED IQBAL
33 SAIQA NOOR MUHAMMAD AMIN
34 AIMEN HAMID
35 KAINAT NOREEN MUNAWAR HUSSAIN
36 AYESHA JAVED JAVED AZIZ G6 In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator of macrophage infiltration and cancer immunotherapy target
37 FATIMA MEHMOOD SHAHID MEHMOOD
38 USHBA MARIA ABDUL BASIT
39 HAFISA MUHAMMAD MUHAMMAD ZUBAIR HAMEED
40 KINZA TUL EMAN SHABBIR AHMAD
41 TAYYABA AWAN MUNIR AHMAD ALVI
42 AMNA RUHTAB MUHAMMAD IRSHAD
43 MARYAM KHALIQ ABDUL KHALIQ G7 Selective Surface PEGylation of UiO-66 Nanoparticles for Enhanced Stability, Cell Uptake, and pH-Responsive Drug Delivery
44 AKASHA SHAH JEHAN SHAH JEHAN
45 SHIZZA SAEED SAEED KHALIQ
46 TASKEEN FATIMA MUHAMMAD RASHEED
47 AREESHA ABID ABID KHURSHID
48 FATIMA SHAHID MUHAMMAD SHAHID
49 FAIZA ZIA ZIA MAJEED
50 ATIKA SHAFAQ ZAHID RAZA G8 Preventing Allograft Rejection by Targeting Immune Metabolism
51 MUHMMAD BILAL SARWAR GHULAM SARWAR
52 EISHA SAHAR MUHAMMAD AQEEL
53 MAHEEN RAZA RAFI AHMAD RAZA RAFI
54 YASHFA FATIMA FAIZ UL HASSAN SHAH
55 HIFZA NOOR TARIQ TARIQ MEHMOOD
56 ALLIHA ASIF ASIF HUSSAIN
57 AMNA  MUHAMMAD ASHRAF G9 Alteration of Tumor Metabolism by CD4+ T Cells Leads to TNF-a-Dependent Intensification of Oxidative Stress and Tumor Cell Death
58 MARIAM ARIF MUHAMMAD ARIF
59 MAHEEN SABIR SABIR HUSSAIN SHAH
60 RAMEEN SHAHZAD SHAHZAD WASIM
61 AYESHA SADDIQA FAISAL IJAZ
62 AYESHA RIAZ MUHAMMAD PERVAIZ
63 SAMA PERVAIZ ZULIFQAR AHMAD PERVAIZ
64 FATIMA NADEEM NADEEM HAIDER G10 Controlling cancer-induced inflammation with a nucleic acid scavenger prevents lung metastasis in murine models of breast cancer
65 ALEEZA FATIMA MUHAMMAD HUSSAIN
66 AYESHA NADEEM MUHAMMAD NADEEM
67 Faiqa Noor  
68 Areeb Rubab  
69 Rimsha Ishtiaq   
70 Izza   
71 Saika noreen  
72 Vaneeza Shahbaz  
73 Sadia Khalid  
74 Sajal Rashad  
75 Eman Shafiq  
76 Farwa Naz  
78 Rimsha Naveed  
79 Zainab Haidar  
80  Faiqa Noor  
81  Areeza Rubab   G11 PRMT6 Regulates RAS/RAF Binding and MEK/ERKMediated Cancer Stemness Activities in Hepatocellular Carcinoma through CRAF Methylation
82    
83    
84 Eman Tayyab 2022-ag-5744
85 Malaika Ajmal 2022-ag-5701
86 Laiba Ghaffar  2022-ag-5703
87 Mahe Mubeen  2022-ag-5739
88 Rabia Yaseen  2022-ag-5746
89 Umm-E-Aime 2022-ag-5760
90 Muhammad Salman 2022-ag-5741
91 Umar Ashraf 2022-ag-5702
92 Awais afzal 2022-ag-5711

BIOCHEM-301                  ELEMENTARY BIOCHEMISTRY                  3(2 -1)

 Course Learning Outcomes

By the end of this course students will be able to;

  1. Students will be able to understand the knowledge of buffers and will also be able to learn its preparation practically.
  2. Students will learn the basic knowledge about enzymes.
  3. Students will be able to understand basics of major biomolecules like carbohydrates, lipids, Proteins and Nucleic acids.
  4. Students will practically learn the qualitative/quantitative analysis of biological molecules that are also nutritional important like carbohydrates, proteins, lipids etc.

 Theory  

A general introduction to the science of biochemistry and its importance. Ionization of water, weak acid and weak bases, Define and describe pH, buffers, diffusion, osmosis and osmotic pressure. Enzymes: Classification, nomenclature, characteristics, coenzymes, cofactors and prosthetic groups. Mechanism of enzyme action. Enzyme inhibition. Carbohydrates: Classification, characteristics, aerobic and anaerobic oxidation of glucose; glycolysis, citric acid cycle, Electron transport chain (ETC) and fermentations, biological functions of carbohydrates. Lipids: Composition and classification, structure of saturated and unsaturated fatty acids and their properties, characteristics of fats and oils, general metabolism of fats and oils. Proteins: Composition and classification, characteristics and classification of amino acids, peptides and level of structural organization of proteins, physiological function and general metabolism of proteins. Nucleic acids: Chemical composition, structure of DNA and RNA and its types. A general overview of central dogma of molecular biology.

 Practical

pH metry and determination of pH value of biological fluids. Preparation of buffers of definite pH. Estimation of optical activity by polarimetry. Qualitative analysis of carbohydrates. Qualitative analysis of urine for normal and abnormal constituents like albumins, acetone bodies and sugar. Estimation of glucose in biological fluids. Determination of acid, saponification and iodine values of fats/oils. Estimation of lactose and casein in milk.

Suggested Readings

  1. Ahmad, M. 2019. Essentials of Medical Biochemistry Vol-I. 9th Ed. Nishtar Publications Press. 10A Model town Multan.
  2. Boyer, R. F. 2012. Biochemistry Laboratory, Modern theory and techniques. 3rd Pearson Education, New Jersey, USA
  3. Murray, R. K., D. A. Bender, K.M. Botham, P. J. Kennelly, V. W. Rodwell and P. A. Weil. 2021. Harper’s Illustrated Biochemistry. 32nd MvGraw Hill, NY, USA
  4. Nelson, D. L. and M. M. Cox. 2021. Lehninger Principles of Biochemistry. 8th WH Freeman & Company, NY, USA
  5. Nigam, A and A. Archana . 2008. Lab manual in Biochemistry, Immunology and Biotechnology. 3rd Ed Tata Mcgraw Hill Publishing Company (Pvt.) Ltd. New Dehli, India
  6. Sawhney, C. K. and R. Singh. 2014. Introductory Practical Biochemistry. 5th Norosa Pub. House, New Dehli, India
  7. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd  edition, ISBN: 978-969-7502-04-
  8. .Ali, M. et al., Experimental Biochemistry and Biotechnology (Series II), 2023. 2nd edition, ISBN: 978-969-7502-05-8.
  9. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series III), 2024, 1st edition, ISBN: 978-969-7502-06-5
MMGL
Life Sciences by Dr. Muhammad Ali
This is template... Try to write, whatever headings are possible for a practical.

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