Biochem-307 Biochemical Analysis
(list of practical)

  1. Preparation of solutions: Preparation of Buffer (2.1, 2.3, 2.7-2.9, 2.11, 2.12, 2.22, 2.24) 
  2. Carbohydrate Analysis: Group Identification Test, Identification of mono, di and polysaccharides, Identification of reducing sugars, Identification of ketose sugar and their confirmation by phenylhydrazine test, Identification scheme for carbohydrates, (3.1-3.8)
  3. Estimation of glucose in blood sample by phenol sulphuric acid method, (3.9, 3.12, 7.3)
  4. Estimation of amino acids, (4.4)
  5. Biuret Test and Ninhydrin Test, (4.11, 4.4)
  6. Precipitation tests of proteins. (5.6)
  7. Identification of lipids (6.1-6.7)
  8. Estimation of vitamin C by DCIP method. (7.9)
  9. Proximate analysis of food/feed samples. (7.1-7.11)

 Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd edition, ISBN: 978-969-7502-04-1td

Mid-term syllabus of Biochem-307:Solution, colloidal and suspension system. pH & its importance and application, Buffers: natural and synthetic buffers, characteristics of buffer, importance and applications. Henderson-Hasselbalch equation.;

…. PLUS…. First 150 MCQs from chapter 2 (150/302) the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.

Final exam syllabus of Biochem-307: Reaction and mechanism of different biochemical analysis (carbohydrates, proteins, lipids and nucleic acids) and their importance. Proximate analysis, importance and applications. plus 25% of mid-term syllabus

 …. PLUS…. First 150 MCQs from each of chapters 2 (150/302) and chapter -3 (150/729) of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam..

……PLUS……… Final exam will contain 30% from mid syllabus.

NO EXCUSE WILL BE ACCEPTED

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2017-ag-9382 Haidar Iftikhar

2017-ag-10077 Ahmad Faraz 

BIOCHEM-307                  BIOCHEMICAL ANALYSIS                  3(1 -2)

 Course Learning Outcomes

By the end of this course students will be able to;

  1. 1. Apply an understanding of the methods and techniques associated to solutions, pH and buffers.
  2. 2. Explain the principles behind major biochemical molecules analysis.
  3.  3. Demonstrate food and feed analysis. 

Theory

Solution, colloidal and suspension system. pH & its importance and application, Buffers: natural and synthetic buffers, characteristics of buffer, importance and applications. Henderson-Hasselbalch equation. Reaction and mechanism of different biochemical analysis (carbohydrates, proteins, lipids and nucleic acids) and their importance. Proximate analysis, importance and applications.

 

 

Practical

Preparation of solutions: Preparation of Buffer, Carbohydrate Analysis: Group Identification Test, Identification of mono, di and polysaccharides, Identification of reducing sugars, Identification of ketose sugar and their confirmation by phenylhydrazine test, Identification scheme for carbohydrates,  Estimation of glucose in blood sample by phenol sulphuric acid method, Estimation of amino acids, Biuret Test and Ninhydrin Test, Precipitation tests of proteins. Identification of lipids. Estimation of vitamin C by DCIP method. Proximate analysis of food/feed samples. 

 

Suggested Readings

1.       Boyer, R.F. 2014. Modern Experimental Biochemistry. 3rd Edition. The Benjamin / Cummings
Publishing Co., USA

2.   Nelson, D.L and M.M. Cox. 2017. Lehninger Principles of Biochemistry. 7th ed. Worth Publishers, New York.

3.    Murray, R., D. Bender, K.M. Botham, P.J. Kennelly, V. Rodwell and P.A. Weil. 2015. Harper’s Illustrated Biochemistry, 30th edition. The McGraw-Hill companies Inc. USA. ISBN-10: 0071825347

4.  Gowenlock, A. H., J.R. McMurray and D.M. McLauchlan. 2006. Varley’s Practical Clinical Biochemistry 6th ed. CBS Publishers and distributors. New Delhi, India.

5.      Sawhney, S.K. and R. Singh. 2009. Introductory Practical Biochemistry. Narosa Publishing House, New Delhi, India.

6.      Shawn, O.F.  and L. E. Taylor. 2009. Biochemistry Lab Manual. Cengage Learning, USA.

7.      Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2019, 3rd edition, ISBN: 978-969-7502-04-1

8.      Ali, M. et al., Experimental Biochemistry and Biotechnology (Series II), 2019. 1st edition, ISBN: 978-969-23317-2-2.

9.      Ali, M. et al., Experimental Biochemistry and Biotechnology (Series III), 2023, 1st edition, ISBN: 978-969-23317-6-0 

Life Sciences by Dr. Muhammad Ali
This is template... Try to write, whatever headings are possible for a practical.
Sr.Ag No.Student’s NameCourse codeTopic of presentation (with one page summary)
12019-ag-5768Muhammad Umer303CH-18 (amino acid oxidation)
22017-ag-6984Aqsa Azam303CH-14 (glycolysis etc.)
32018-ag-6385Saira Akmal511Transcription, translation in eukaryotes
42021-ag-8641Madeeha Manzoor307Henderson-Hasselbalch equation
52021-ag-8660M Salik Wali307Proximate analysis
62019-ag-9479Gulfam Shahzada307pH & its importance and application

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