Biochem-514, Biochemical Techniques, 3(1-2)
(list of practical)

  1. Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; (4.10)
  2. Prove Beer’s law by creating standard graph between absorbance and concentration; (4.13)
  3.  Demonstration about diffusion, dialysis and osmosis; (2.25, 2.26)
  4. Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; (4.4, 4.8)
  5. Carbohydrate analysis and estimation of glucose by spectrophotometer; (3.9)
  6. Total protein estimation by Biuret/Bradford and UV method; (4.10, 4.11, 4.14)
  7. Cholesterol/sterol estimation by LB reagent method; (6.13)
  8. Proximate analysis of foods and feed; (7.1 to 7.11)
  9. Glycogen: Isolation and characterization; (3.16, 3.17)
  10. Electrophoresis of proteins/nucleic acids. (4.16)
Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd  edition, ISBN: 978-969-7502-04-1.

Mid-term syllabus of Biochem-514, 3(1-2):

Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures; Laboratory glassware and basic instrumentation; …….PLUS….. experiment 2.1 (how will you prepare 50 mL of 0.2 molar solution of glucose?).

…. PLUS…. First 150 MCQs from chapters 2 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.

 Final syllabus of Biochem-514:

…. PLUS…. 30% of final paper will contain syllabus of mid

…. PLUS…. Standard procedures: Preparation and storage of reagents. Donnan’s equilibrium; Theoretical background of chromatography; Basics of electrophoresis; Importance of biochemical analysis in plant and animal sciences.

…. PLUS…. First 150 MCQs of each of chapter 2,3 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters may also appear in the exam.

 Assignemtns and presentations of Biochem-514: 

  1. The weightage of the assignment and presentation will be decided later. 
  2. Start preparing your presentations and assignments….. Assignment submission is 18-04-2023.
  3. The presentations will also be started right after the mid-term exam.
  4. PPT file should not contain any designing/colours other than figures and diagrams.
  5. Slides should be in 16X9 ratio.
  6. Fonts of all header should be same and of all other text should be same. 
  7. For detailed guidelines about the presentation of research article, visit: https://www.lifesciencesorg.combiochem-720/
  8. Download the template ppt file to make your presentation. The link is here (copy this link and paste in a new tab).
  9. Strictly follow the ppt format and it is better if you write and paste your material in the same ppt file.

NO EXCUSE WILL BE ACCEPTED

 

K. General Question to be asked during presentation

1) Pathway or importance of gene/technology/discovery

2) Rationale

3) Conclusion of the full article

4) Conclusion of every figure

5) New findings presented in the article

6) Applications of the findings

7) Future prospective

8) Applications of the work

9) Principle of every technique used in the research article

Moreover, provide me a printed summary of your article containing the above headings given under ‘K’.

L. During Presentations, for EVERY FIGURE and graph, we may ask:

1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively

__________________________________________________________________________________

Link to download the book ( MCQs in Biochemistry) is given below:

https://b-ok.cc/dl/1055171/2292c5


__________________________________________________________________________________

Microsoft Teams meeting
Join on your computer, mobile app or room device

Click here to join the meeting

Meeting ID: 316 824 288 013
Passcode: WNyjeA

 __________________________________________________________________________________
 

Microsoft teams will be used whenever online classes will be held. You must make your first name as your registration number.

2017-ag-9382 Haidar Iftikhar

2017-ag-10077 Ahmad Faraz 

 

L. During Presentations, for EVERY FIGURE and graph, we may ask:

1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively

  1. BIOCHEM-514        Biochemical Techniques            3(1-2)

 Course Learning Outcomes

By the end of this course students will be able to;

  1. 1.       Demonstrate lab. safety related knowledge and protocols, alongwith general lab tools and instruments
  2. 2.       Relate advance knowledge of chromatography, dialysis, photometry, electrophoresis in the analysis of biomolecules
  3. 3.       Estimate and identify biomolecules via chromatography, colorimeter/spectrophotometer, titrations etc.
  4. 4.       Analyse food and feed samples and learn electrophoresis

Theory

Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures; Laboratory glassware and basic instrumentation; Standard procedures: Preparation and storage of reagents. Donnan’s equilibrium; Theoretical background of chromatography; Basics of electrophoresis; Importance of biochemical analysis in plant and animal sciences.

 

Practical

Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; Prove Beer’s law by creating standard graph between absorbance and concentration; Demonstration about diffusion, dialysis and osmosis; Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; Carbohydrate analysis and estimation of glucose by spectrophotometer; True protein estimation by Biuret/Bradford and UV method; Cholesterol/sterol estimation by LB reagent method; Proximate analysis of foods and feed; Glycogen: Isolation and characterization; Electrophoresis of proteins/nucleic acids.

 

Suggested Readings

  1. 1. Boyer, R.F. 2014. Modern Experimental Biochemistry. 3rd Ed. The Benjamin/Cummings Publishing Co., USA.
  2. 2. Kaushik, G.G., N. Sharma, S. Dabeer and R. Jindal. 2020. Practical Manual of Biochemistry. CBS Publishers & Distributors Pvt Ltd, India.
  3. 3. Sawhney, S.K. and R. Singh. 2014. Introductory Practical Biochemistry. Narosa Publishing House, New Delhi, India.
  4. 4. Shawn, O.F. and L.E. Taylor. 2009. Biochemistry Lab Manual. Cengage Learning, USA.
  5. 5. Varley, H., A.H. Gowenlock, J.R. McMurray and D.M. McLauchlan. 2006. Varley’s Practical Clinical Biochemistry. 6th Ed. CBS Publishers and distributors. New Delhi, India.
  6.  Wilson, K. and J. Walker. 2018. Practical Biochemistry: Principles and Techniques. 8th Ed. Cambridge University Press, UK.
  7. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd  edition, ISBN: 978-969-7502-04-1.
  8. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series II), 2023. 2nd edition, ISBN: 978-969-7502-05-8.
  9. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series III), 2024, 1st edition, ISBN: 978-969-7502-06-5.

BS Biochemistry, Biovhem-514, Winter 2025-26, Dr. Muhammad Ali

 

Articles for presentations:

https://drive.google.com/drive/folders/1elpSAZaYhPgCEt1aSXOUZTcVijkAyvPx?usp=sharingAli

 

     
Sr.Ag No.Student’s NameGroupFor presentation and 1 page summary
12023-ag-8291Esha TallatG11. Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; (4.10)
22023-ag-8292Aisha Afzal
32023-ag-8293Amina Tariq
42023-ag-8294Zunaira Fatima
52023-ag-8295SamreenG22. Prove Beer’s law by creating standard graph between absorbance and concentration; (4.13)
62023-ag-8296Hira Siddique
72023-ag-8297Nudrat Batool
82023-ag-8298Laiba AmjadG33.  Demonstration about diffusion, dialysis and osmosis; (2.25, 2.26)
92023-ag-8299Fizzah Batool
102023-ag-8300Ayesha Tariq
112023-ag-8301Hunaina Javed
122023-ag-8302Amna AnjumG44. Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; (4.4, 4.8)
132023-ag-8303Alweera Gull
142023-ag-8304Iqra Qousain
152023-ag-8306Samreen ShahzadiG55. Carbohydrate analysis and estimation of glucose by spectrophotometer; (3.9)
162023-ag-8307Kashmala Sajid
172023-ag-8309Ammara
182023-ag-8311Aital Fatima
192023-ag-8312Maroosha HateemG66. Total protein estimation by Biuret/Bradford and UV method; (4.10, 4.11, 4.14)
202023-ag-8313Taram Ashiq
212023-ag-8315Noor Fatima
222023-ag-8316Malaika ArshadG77. Cholesterol/sterol estimation by LB reagent method; (6.13)
232023-ag-8317Aleeza Jameel
242023-ag-8319Mehreen Amjad
252023-ag-8320Ayesha Sarwar
262023-ag-8321Vidad Ajaz ChG88. Proximate analysis of foods and feed; (7.1 to 7.11)
272023-ag-8323Arooj Fatima
282023-ag-8324Eman Fatima
292023-ag-8325Barrirah GhaffarG99. Glycogen: Isolation and characterization; (3.16, 3.17)
302023-ag-8326Malaika Arshad
312023-ag-8327Saleh Khatoon
322023-ag-8329Sawaira Qummer
332023-ag-8330Abdullah NisarG1010. Electrophoresis of proteins/nucleic acids. (4.16)
342023-ag-8332Tuba Ghazanfar
352022-ag-5534Aqsa Niamat
362023-ag-9078Mehak
372023-ag- 
382023-ag- 

 

MMGL
This is template... Try to write, whatever headings are possible for a practical.
Life Sciences by Dr. Muhammad Ali
This is template... Try to write, whatever headings are possible for a practical.