Mid-term, syllabus of Biochem-513: Structural organization of genes and chromosomes in prokaryotes and eukaryotes, nucleosomes, properties of DNA and RNA in solution; Nucleic acid stability; DNA Supercoiling; Replication of DNA: Theory, semi-conservative replication and molecular mechanism of replication in prokaryotes and eukaryotes; Enzymology of DNA replication; Molecular nature of mutations, DNA damage and repair; SOS response; Genetic recombination and mobile genetic elements;.
…. PLUS…. First 200 MCQs from chapters 9 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.
Final exam, syllabus of Biochem-513: Transcription: Promoters and enhancer elements, RNA synthesis in prokaryotes and eukaryotes, tRNA, mRNA and rRNA processing, capping, splicing and polyadenylation of eukaryotic mRNA; Reverse transcription; RNA replication; Genetic code and Wobble hypothesis; Translation, essential factors, enzymes, initiation, elongation and termination of protein synthesis; Post-translational modifications and targeting of proteins; Control of transcription and translation; Regulation of gene expression in prokaryotes; lac and trp operons; Overview of molecular biology techniques; DNA restriction and modification; Advances in biotechnology and genetic engineering.
…. PLUS…. First 200 MCQs from chapters 9 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in exam. Mid term syllabus will be included in final exam.
Assignments and presentations:
- The weightage of the assignment and presentation will be decided later.
- Start preparing your presentations and assignments.
- The presentations will also be started right after the mid-term exam.
- PPT file should not contain any designing/colours other than figures and diagrams.
- Slides should be in 16X9 ratio.
- Fonts of all header should be same and of all other text should be same.
- For detailed guidelines about the presentation of research article, visit: https://www.lifesciencesorg.combiochem-720/
- Download the template ppt file to make your presentation. The link is here (copy this link and paste in a new tab).
- Strictly follow the ppt format and it is better if you write and paste your material in the same ppt file.
K. General Question to be asked during presentation
- 1) Pathway or importance of gene/technology/discovery
- 2) Rationale
- 3) Conclusion of the full article
- 4) Conclusion of every figure
- 5) New findings presented in the article
- 6) Applications of the findings
- 7) Future prospective
- 8) Applications of the work
- 9) Principle of every technique used in the research article
Moreover, provide me a printed summary of your article containing the above headings given under ‘K’.
L. During Presentations, for EVERY FIGURE and graph, we may ask:
1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively
NO EXCUSE WILL BE ACCEPTED
_______________________________________________________________
Link to download the book ( MCQs in Biochemistry) is given below:
https://b-ok.cc/dl/1055171/2292c5
Meeting ID: 336 479 908 561
Passcode: aH9EDN
_______________________________________________________________
Microsoft teams will be used whenever online classes will be held. You must make your teams name as follows.
2017-ag-9382 Haidar Iftikhar
2017-ag-10077 Ahmad Faraz
BIOCHEM-513 MOLECULAR BIOCHEMISTRY 3(3-0)
Course Learning Outcomes
By the end of this course students will be able to;
- Describe the basic knowledge and concepts of molecular biology.
- Demonstrate the process of DNA replication, DNA damage and repair, transcription and translation.
- Know basic concepts of gene expression and genetic engineering.
Course Contents
Theory
Structural organization of genes and chromosomes in prokaryotes and eukaryotes, nucleosomes, properties of DNA and RNA in solution; Nucleic acid stability; DNA Supercoiling; Replication of DNA: Theory, semi-conservative replication and molecular mechanism of replication in prokaryotes and eukaryotes; Enzymology of DNA replication; Molecular nature of mutations, DNA damage and repair; SOS response; Genetic recombination and mobile genetic elements; Transcription: Promoters and enhancer elements, RNA synthesis in prokaryotes and eukaryotes, tRNA, mRNA and rRNA processing, capping, splicing and polyadenylation of eukaryotic mRNA; Reverse transcription; RNA replication; Genetic code and Wobble hypothesis; Translation, essential factors, enzymes, initiation, elongation and termination of protein synthesis; Post-translational modifications and targeting of proteins; Control of transcription and translation; Regulation of gene expression in prokaryotes; lac and trp operons; Overview of molecular biology techniques; DNA restriction and modification; Advances in biotechnology and genetic engineering.
Suggested Readings
- Molecular Cell Biology -Lodish 8e (major book for this course)
- Molecular Biology-Weaver-5e (major book for this course)
- Nelson, D.L and M.M. Cox. 2017. Lehninger Principles of Biochemistry. 7th ed. Worth Publishers, New York, NY, USA.
- Ferrier, D. R. 2017. Biochemistry: Lippincott’s Illustrated Reviews. 7th ed. Lippincott Williams and Wilkins. U.S.A.
- Brown, T., 2016. Gene Cloning and DNA Analysis. 7th Edition. Blackwell Scientific Publications, Melbourne. ISBN-10 # 1119072565.
- Sambrook, J. F., Russell, D. W. and Irwin, N. 2000. Molecular cloning: A laboratory manual, 3rd ed. Cold Spring Harbor Laboratory press, Cold Spring Harbor, New York, NY, USA.
- Voet, D., Voet, J.G and C.W. Pratt. 2013. Fundamentals of Biochemistry, Life at the Molecular Level. 4th ed. John Wiley & Sons. Inc. New York, NY, USA.
- Weaver, R. F. 2012. Molecular Biology. 5th ed. McGraw Hill Higher Education.,
| Biochem-513, BS Zoology, 6M–Spring 2026, Dr. Muhammad Ali; 09-02-2026 | ||||
| Sr. | Ag No. | Student’s Name | Group | Assignment for group discussion and for making PPT according to template, one page summary and answers of 9 questions and presentation in the classResearch article for presentation and 1 page summary |
| 1 | 2022-ag-5534 | AQSA NIAMAT | G1 | Article 21 |
| 2 | 2023-ag-9078 | MEHAK IMRAN | ||
| 3 | 2023-ag-8291 | ESHA TALLAT | ||
| 4 | 2023-ag-8292 | AISHA AFZAL | ||
| 5 | 2023-ag-8293 | AMINA TARIQ | ||
| 6 | 2023-ag-8294 | ZUNAIRA FATIMA | G2 | Article 22 |
| 7 | 2023-ag-8295 | SAMREEN BUX | ||
| 8 | 2023-ag-8296 | HIRA SIDDIQUE | ||
| 9 | 2023-ag-8297 | NUDRAT BATOOL | ||
| 10 | 2023-ag-8299 | FIZZAH BATOOL | ||
| 11 | 2023-ag-8300 | AYESHA TARIQ | G3 | Article 23 |
| 12 | 2023-ag-8301 | HUNAINA JAVED | ||
| 13 | 2023-ag-8302 | AMNA ANJUM | ||
| 14 | 2023-ag-8303 | ALWEERA GULL | ||
| 15 | 2023-ag-8304 | IQRA QOUSAIN | ||
| 16 | 2023-ag-8306 | SAMREEN SHAHZADI | G4 | Article 24 |
| 17 | 2023-ag-8307 | KASHMALA SAJID | ||
| 18 | 2023-ag-8309 | AMMARA KARAM | ||
| 19 | 2023-ag-8311 | AITAL FATIMA | ||
| 20 | 2023-ag-8312 | MAROOSHA HATEEM | ||
| 21 | 2023-ag-8313 | TARAM ASHIQ | G5 | Article 25 |
| 22 | 2023-ag-8315 | NOOR FATIMA | ||
| 23 | 2023-ag-8316 | MALAIKA ARSHAD | ||
| 24 | 2023-ag-8317 | ALEEZA JAMEEL | ||
| 25 | 2023-ag-8319 | MEHREEN AMJAD | ||
| 26 | 2023-ag-8320 | AYESHA SARWAR | G6 | Article 26 |
| 27 | 2023-ag-8321 | VIDAD AJAZ CH. | ||
| 28 | 2023-ag-8324 | EMAN FATIMA | ||
| 29 | 2023-ag-8325 | BARRIRAH GHAFFAR | ||
| 30 | 2023-ag-8326 | MALAIKA ARSHAD | ||
| 31 | 2023-ag-8327 | SALEH KHATOON | G7 | Article 27 |
| 32 | 2023-ag-8329 | SAWAIRA QUMMER | ||
| 33 | 2023-ag-8330 | ABDULLAH NISAR | ||
| 34 | 2023-ag-8332 | TUBA GHAZANFAR | ||
| 35 | ||||
