Mid-term, syllabus of Biochem-513:  Structural organization of genes and chromosomes in prokaryotes and eukaryotes, nucleosomes, properties of DNA and RNA in solution; Nucleic acid stability; DNA Supercoiling; Replication of DNA: Theory, semi-conservative replication and molecular mechanism of replication in prokaryotes and eukaryotes; Enzymology of DNA replication; Molecular nature of mutations, DNA damage and repair; SOS response; Genetic recombination and mobile genetic elements;.  

…. PLUS…. First 200 MCQs from chapters 9 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.

Final exam, syllabus of Biochem-513: Transcription: Promoters and enhancer elements, RNA synthesis in prokaryotes and eukaryotes, tRNA, mRNA and rRNA processing, capping, splicing and polyadenylation of eukaryotic mRNA; Reverse transcription; RNA replication; Genetic code and Wobble hypothesis; Translation, essential factors, enzymes, initiation, elongation and termination of protein synthesis; Post-translational modifications and targeting of proteins; Control of transcription and translation; Regulation of gene expression in prokaryotes; lac and trp operons; Overview of molecular biology techniques; DNA restriction and modification; Advances in biotechnology and genetic engineering. 

…. PLUS…. First 200 MCQs from chapters  9 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in exam. Mid term syllabus will be included in final exam.

Assignments and presentations: 

  1. The weightage of the assignment and presentation will be decided later. 
  2. Start preparing your presentations and assignments.
  3. The presentations will also be started right after the mid-term exam.
  4. PPT file should not contain any designing/colours other than figures and diagrams.
  5. Slides should be in 16X9 ratio.
  6. Fonts of all header should be same and of all other text should be same. 
  7. For detailed guidelines about the presentation of research article, visit: https://www.lifesciencesorg.combiochem-720/
  8. Download the template ppt file to make your presentation. The link is here (copy this link and paste in a new tab).
  9. Strictly follow the ppt format and it is better if you write and paste your material in the same ppt file.

K. General Question to be asked during presentation

  1. 1) Pathway or importance of gene/technology/discovery
  2. 2) Rationale
  3. 3) Conclusion of the full article
  4. 4) Conclusion of every figure
  5. 5) New findings presented in the article
  6. 6) Applications of the findings
  7. 7) Future prospective
  8. 8) Applications of the work
  9. 9) Principle of every technique used in the research article

Moreover, provide me a printed summary of your article containing the above headings given under ‘K’.

L. During Presentations, for EVERY FIGURE and graph, we may ask:

1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively

NO EXCUSE WILL BE ACCEPTED

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Link to download the book ( MCQs in Biochemistry) is given below:

https://b-ok.cc/dl/1055171/2292c5


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Microsoft Teams meeting
Join on your computer, mobile app or room device

Click here to join the meeting

Meeting ID: 336 479 908 561
Passcode: aH9EDN

_______________________________________________________________

Microsoft teams will be used whenever online classes will be held. You must make your teams name as follows. 

2017-ag-9382 Haidar Iftikhar

2017-ag-10077 Ahmad Faraz

BIOCHEM-513                     MOLECULAR BIOCHEMISTRY                                    3(3-0) 

Course Learning Outcomes

By the end of this course students will be able to;

  1. Describe the basic knowledge and concepts of molecular biology.
  2. Demonstrate the process of DNA replication, DNA damage and repair, transcription and translation.
  3. Know basic concepts of gene expression and genetic engineering.

 

Course Contents

 Theory

Structural organization of genes and chromosomes in prokaryotes and eukaryotes, nucleosomes, properties of DNA and RNA in solution; Nucleic acid stability; DNA Supercoiling; Replication of DNA: Theory, semi-conservative replication and molecular mechanism of replication in prokaryotes and eukaryotes; Enzymology of DNA replication; Molecular nature of mutations, DNA damage and repair; SOS response; Genetic recombination and mobile genetic elements; Transcription: Promoters and enhancer elements, RNA synthesis in prokaryotes and eukaryotes, tRNA, mRNA and rRNA processing, capping, splicing and polyadenylation of eukaryotic mRNA; Reverse transcription; RNA replication; Genetic code and Wobble hypothesis; Translation, essential factors, enzymes, initiation, elongation and termination of protein synthesis; Post-translational modifications and targeting of proteins; Control of transcription and translation; Regulation of gene expression in prokaryotes; lac and trp operons; Overview of molecular biology techniques; DNA restriction and modification; Advances in biotechnology and genetic engineering. 

 Suggested Readings

  1. Molecular Cell Biology -Lodish 8e (major book for this course)
  2. Molecular Biology-Weaver-5e (major book for this course)
  3. Nelson, D.L and M.M. Cox. 2017. Lehninger Principles of Biochemistry. 7th ed. Worth Publishers, New York, NY, USA. 
  4.  Ferrier, D. R. 2017. Biochemistry: Lippincott’s Illustrated Reviews. 7th ed.  Lippincott Williams and Wilkins. U.S.A.
  5. Brown, T., 2016. Gene Cloning and DNA Analysis. 7th Edition. Blackwell Scientific Publications, Melbourne. ISBN-10 # 1119072565.
  6.  Sambrook, J. F., Russell, D. W. and Irwin, N. 2000. Molecular cloning: A laboratory manual, 3rd ed. Cold Spring Harbor Laboratory press, Cold Spring Harbor, New York, NY, USA. 
  7. Voet, D., Voet, J.G and C.W. Pratt. 2013. Fundamentals of Biochemistry, Life at the Molecular Level. 4th ed. John Wiley & Sons. Inc. New York, NY, USA.
  8. Weaver, R. F. 2012. Molecular Biology. 5th ed. McGraw Hill Higher Education.,
Biochem-513, BS Zoology, 6M–Spring 2026, Dr. Muhammad Ali; 09-02-2026
         
Sr. Ag No. Student’s Name Group Assignment
for group discussion and for making PPT according to template, one page
summary and answers of 9 questions and presentation in the classResearch
article for presentation and 1 page summary
1 2022-ag-5534 AQSA NIAMAT G1 Article 21
2 2023-ag-9078 MEHAK IMRAN
3 2023-ag-8291 ESHA TALLAT
4 2023-ag-8292 AISHA AFZAL 
5 2023-ag-8293 AMINA TARIQ
6 2023-ag-8294 ZUNAIRA FATIMA G2 Article 22
7 2023-ag-8295 SAMREEN BUX
8 2023-ag-8296 HIRA SIDDIQUE
9 2023-ag-8297 NUDRAT BATOOL
10 2023-ag-8299 FIZZAH BATOOL
11 2023-ag-8300 AYESHA TARIQ G3 Article 23
12 2023-ag-8301 HUNAINA JAVED
13 2023-ag-8302 AMNA ANJUM
14 2023-ag-8303 ALWEERA GULL
15 2023-ag-8304 IQRA QOUSAIN
16 2023-ag-8306 SAMREEN SHAHZADI G4 Article 24
17 2023-ag-8307 KASHMALA SAJID
18 2023-ag-8309 AMMARA KARAM
19 2023-ag-8311 AITAL FATIMA
20 2023-ag-8312 MAROOSHA HATEEM
21 2023-ag-8313 TARAM ASHIQ G5 Article 25
22 2023-ag-8315 NOOR FATIMA
23 2023-ag-8316 MALAIKA ARSHAD
24 2023-ag-8317 ALEEZA JAMEEL
25 2023-ag-8319 MEHREEN AMJAD
26 2023-ag-8320 AYESHA SARWAR G6 Article 26
27 2023-ag-8321 VIDAD AJAZ CH.
28 2023-ag-8324 EMAN FATIMA
29 2023-ag-8325 BARRIRAH GHAFFAR
30 2023-ag-8326 MALAIKA ARSHAD
31 2023-ag-8327 SALEH KHATOON G7 Article 27
32 2023-ag-8329 SAWAIRA QUMMER
33 2023-ag-8330 ABDULLAH NISAR
34 2023-ag-8332 TUBA GHAZANFAR 
35