Biochem-514, Biochemical Techniques, 3(1-2)
(list of practical)

  1. Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; (4.10)
  2. Prove Beer’s law by creating standard graph between absorbance and concentration; (4.13)
  3.  Demonstration about diffusion, dialysis and osmosis; (2.25, 2.26)
  4. Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; (4.4, 4.8)
  5. Carbohydrate analysis and estimation of glucose by spectrophotometer; (3.9)
  6. Total protein estimation by Biuret/Bradford and UV method; (4.10, 4.11, 4.14)
  7. Cholesterol/sterol estimation by LB reagent method; (6.13)
  8. Proximate analysis of foods and feed; (7.1 to 7.11)
  9. Glycogen: Isolation and characterization; (3.16, 3.17)
  10. Electrophoresis of proteins/nucleic acids. (4.16)
Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd  edition, ISBN: 978-969-7502-04-1.

Mid-term syllabus of Biochem-514, 3(1-2):

Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures; Laboratory glassware and basic instrumentation; …….PLUS….. experiment 2.1 (how will you prepare 50 mL of 0.2 molar solution of glucose?).

…. PLUS…. First 150 MCQs from chapters 2 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters will also appear in the exam.

 Final syllabus of Biochem-514:

…. PLUS…. 30% of final paper will contain syllabus of mid

…. PLUS…. Standard procedures: Preparation and storage of reagents. Donnan’s equilibrium; Theoretical background of chromatography; Basics of electrophoresis; Importance of biochemical analysis in plant and animal sciences.

…. PLUS…. First 150 MCQs of each of chapter 2,3 of the book ” MCQs in Biochemistry”. MCQs from the taught concepts and book chapters may also appear in the exam.

 Assignemtns and presentations of Biochem-514: 

  1. The weightage of the assignment and presentation will be decided later. 
  2. Start preparing your presentations and assignments….. Assignment submission is 18-04-2023.
  3. The presentations will also be started right after the mid-term exam.
  4. PPT file should not contain any designing/colours other than figures and diagrams.
  5. Slides should be in 16X9 ratio.
  6. Fonts of all header should be same and of all other text should be same. 
  7. For detailed guidelines about the presentation of research article, visit: https://www.lifesciencesorg.combiochem-720/
  8. Download the template ppt file to make your presentation. The link is here (copy this link and paste in a new tab).
  9. Strictly follow the ppt format and it is better if you write and paste your material in the same ppt file.

NO EXCUSE WILL BE ACCEPTED

 

K. General Question to be asked during presentation

1) Pathway or importance of gene/technology/discovery

2) Rationale

3) Conclusion of the full article

4) Conclusion of every figure

5) New findings presented in the article

6) Applications of the findings

7) Future prospective

8) Applications of the work

9) Principle of every technique used in the research article

Moreover, provide me a printed summary of your article containing the above headings given under ‘K’.

L. During Presentations, for EVERY FIGURE and graph, we may ask:

1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively

__________________________________________________________________________________

Link to download the book ( MCQs in Biochemistry) is given below:

https://b-ok.cc/dl/1055171/2292c5


__________________________________________________________________________________

Microsoft Teams meeting
Join on your computer, mobile app or room device

Click here to join the meeting

Meeting ID: 316 824 288 013
Passcode: WNyjeA

 __________________________________________________________________________________
 

Microsoft teams will be used whenever online classes will be held. You must make your first name as your registration number.

2017-ag-9382 Haidar Iftikhar

2017-ag-10077 Ahmad Faraz 

 

L. During Presentations, for EVERY FIGURE and graph, we may ask:

1. The Principle of the experiment.
2. The Reason for doing the experiment.
3. The Physiological importance of the figure.
4. The Conclusions of the figure
5. Prepare well objectively and subjectively

  1. BIOCHEM-514        Biochemical Techniques            3(1-2)

 Course Learning Outcomes

By the end of this course students will be able to;

  1. 1.       Demonstrate lab. safety related knowledge and protocols, alongwith general lab tools and instruments
  2. 2.       Relate advance knowledge of chromatography, dialysis, photometry, electrophoresis in the analysis of biomolecules
  3. 3.       Estimate and identify biomolecules via chromatography, colorimeter/spectrophotometer, titrations etc.
  4. 4.       Analyse food and feed samples and learn electrophoresis

Theory

Good lab practices. Laboratory safety: Hazards in biochemistry laboratory, general safety measures, chemical, physical and biological hazards, spillage and waste disposal, radiation safety and first aid. General laboratory procedures; Laboratory glassware and basic instrumentation; Standard procedures: Preparation and storage of reagents. Donnan’s equilibrium; Theoretical background of chromatography; Basics of electrophoresis; Importance of biochemical analysis in plant and animal sciences.

 

Practical

Study principles of spectrophotometry/colorimetry by preparing absorption spectra of any given sample; Prove Beer’s law by creating standard graph between absorbance and concentration; Demonstration about diffusion, dialysis and osmosis; Chromatography: Paper chromatography, one dimensional two dimensional, circular and column chromatography; Carbohydrate analysis and estimation of glucose by spectrophotometer; True protein estimation by Biuret/Bradford and UV method; Cholesterol/sterol estimation by LB reagent method; Proximate analysis of foods and feed; Glycogen: Isolation and characterization; Electrophoresis of proteins/nucleic acids.

 

Suggested Readings

  1. 1. Boyer, R.F. 2014. Modern Experimental Biochemistry. 3rd Ed. The Benjamin/Cummings Publishing Co., USA.
  2. 2. Kaushik, G.G., N. Sharma, S. Dabeer and R. Jindal. 2020. Practical Manual of Biochemistry. CBS Publishers & Distributors Pvt Ltd, India.
  3. 3. Sawhney, S.K. and R. Singh. 2014. Introductory Practical Biochemistry. Narosa Publishing House, New Delhi, India.
  4. 4. Shawn, O.F. and L.E. Taylor. 2009. Biochemistry Lab Manual. Cengage Learning, USA.
  5. 5. Varley, H., A.H. Gowenlock, J.R. McMurray and D.M. McLauchlan. 2006. Varley’s Practical Clinical Biochemistry. 6th Ed. CBS Publishers and distributors. New Delhi, India.
  6.  Wilson, K. and J. Walker. 2018. Practical Biochemistry: Principles and Techniques. 8th Ed. Cambridge University Press, UK.
  7. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2023, 3rd  edition, ISBN: 978-969-7502-04-1.
  8. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series II), 2023. 2nd edition, ISBN: 978-969-7502-05-8.
  9. Ali, M. et al., Experimental Biochemistry and Biotechnology (Series III), 2024, 1st edition, ISBN: 978-969-7502-06-5.

BS Biochemistry, Biovhem-514, Winter 2025-26, Dr. Muhammad Ali

 

Articles for presentations:

https://drive.google.com/drive/folders/1elpSAZaYhPgCEt1aSXOUZTcVijkAyvPx?usp=sharingAli

 

Sr.

Ag No.

Student’s Name

Group

Research article for presentation and 1 page summary

1

2023-ag-8497

AYEZA ABBAS

G1

PPT of article 11

2

2023-ag-8498

MISBAH IMTIAZ

3

2023-ag-8499

MAH JABEEN

4

2023-ag-8500

LAIBA MOHSIN

5

2023-ag-8501

LAIBA IKRAM

6

2023-ag-8502

SEHRISH AKBAR

G2

PPT of article 12

7

2023-ag-8503

INFAL NASIR

8

2023-ag-8504

ASMA ALTAF

9

2023-ag-8505

IMAN NASIR

10

2023-ag-8506

ANEEZA FATIMA

11

2023-ag-8507

NOOR FATIMA IDREES

G3

PPT of article 13

12

2023-ag-8508

LAIBA ALI

13

2023-ag-8509

EMAN KANWAL

14

2023-ag-8510

AREEBA MALIK

15

2023-ag-8511

TANZEELA AMEEN

16

2023-ag-8512

NOOR FATIMA

G4

PPT of article 14

17

2023-ag-8513

HALEEMA AMIR

18

2023-ag-8514

ZUHAA IMRAN

19

2023-ag-8515

MAIDA MANZOOR

20

2023-ag-8516

FARWA KAMRAN

21

2023-ag-8517

TARBEEA KASAM

G5

PPT of article 15

22

2023-ag-8518

VANEEZA SALEEM

23

2023-ag-8520

ROBINA SAJJAD

24

2023-ag-8521

KIRAN IRSHAD

25

2023-ag-8522

AARFA

26

2023-ag-8523

HINA FATIMA

G6

PPT of article 16

27

2023-ag-8524

IQRA TALIB

28

2023-ag-8525

TAHREEM ASGHAR

29

2023-ag-8526

AMNA AHMAD

30

2023-ag-8527

ARFA RIAZ

31

2023-ag-8528

AENA ABUBAKAR

G7

PPT of article 17

32

2023-ag-8529

AMNA AMEEN

33

2023-ag-8530

MOMNA SHAHZAD

34

2023-ag-8532

SEHAR ANDLEEB ASLAM

35

2023-ag-8533

FAIQA SADAQAT

36

2023-ag-8534

AQSA ASHIQ

G8

PPT of article 18

37

2023-ag-8536

HAMNA IDREES

38

2023-ag-8537

MISBAH AZHAR

39

2023-ag-8538

AFIA NISAR

40

2023-ag-8539

FATIMA IHSAN

41

2023-ag-8541

SEHRISH BATOOL

G9

PPT of article 19

42

2023-ag-8542

MEMOONA BIBI

43

2023-ag-8543

DOSHAL LIAQAT

44

2023-ag-8544

TRABI TARA

45

2023-ag-8545

TAYYABA SAGHEER

46

2023-ag-8546

LAIBA SAEED

G10

PPT of article 20

47

2023-ag-8547

RIMSHA

48

2023-ag-8548

PAKIZA ASHFAQ

49

2023-ag-8564

UME HABIBA

50

2023-ag-8565

MAHNOOR ZAKIR

51

2023-ag-8567

FAIQA

MMGL
This is template... Try to write, whatever headings are possible for a practical.
Life Sciences by Dr. Muhammad Ali
This is template... Try to write, whatever headings are possible for a practical.