BIOCHEM-307                  BIOCHEMICAL ANALYSIS                  3(1 -2)

 Course Learning Outcomes

By the end of this course students will be able to;

1. 1. Apply an understanding of the methods and techniques associated to solutions, pH and buffers.
2. 2. Explain the principles behind major biochemical molecules analysis.
3.  3. Demonstrate food and feed analysis. 

Theory

Solution, colloidal and suspension system. pH & its importance and application, Buffers: natural and synthetic buffers, characteristics of buffer, importance and applications. Henderson-Hasselbalch equation. Reaction and mechanism of different biochemical analysis (carbohydrates, proteins, lipids and nucleic acids) and their importance. Proximate analysis, importance and applications.

Practical

Preparation of solutions: Preparation of Buffer, Carbohydrate Analysis: Group Identification Test, Identification of mono, di and polysaccharides, Identification of reducing sugars, Identification of ketose sugar and their confirmation by phenylhydrazine test, Identification scheme for carbohydrates,  Estimation of glucose in blood sample by phenol sulphuric acid method, Estimation of amino acids, Biuret Test and Ninhydrin Test, Precipitation tests of proteins. Identification of lipids. Estimation of vitamin C by DCIP method. Proximate analysis of food/feed samples. 

Suggested Readings

1.       Boyer, R.F. 2014. Modern Experimental Biochemistry. 3^rd Edition. The Benjamin / Cummings
Publishing Co., USA

2.   Nelson, D.L and M.M. Cox. 2017. Lehninger Principles of Biochemistry. 7th ed. Worth Publishers, New York.

3.    Murray, R., D. Bender, K.M. Botham, P.J. Kennelly, V. Rodwell and P.A. Weil. 2015. Harper’s Illustrated Biochemistry, 30^th edition. The McGraw-Hill companies Inc. USA. ISBN-10: 0071825347

4.  Gowenlock, A. H., J.R. McMurray and D.M. McLauchlan. 2006. Varley’s Practical Clinical Biochemistry 6^th ed. CBS Publishers and distributors. New Delhi, India.

5.      Sawhney, S.K. and R. Singh. 2009. Introductory Practical Biochemistry. Narosa Publishing House, New Delhi, India.

6.      Shawn, O.F.  and L. E. Taylor. 2009. Biochemistry Lab Manual. Cengage Learning, USA.

7.      Ali, M. et al., Experimental Biochemistry and Biotechnology (Series I), 2019, 3^rd edition, ISBN: 978-969-7502-04-1

8.      Ali, M. et al., Experimental Biochemistry and Biotechnology (Series II), 2019. 1^st edition, ISBN: 978-969-23317-2-2.

9.      Ali, M. et al., Experimental Biochemistry and Biotechnology (Series III), 2023, 1^st edition, ISBN: 978-969-23317-6-0